Source:http://linkedlifedata.com/resource/pubmed/id/21836494
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
2011-8-15
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| pubmed:abstractText |
Human papillomavirus (HPV) is established as causative in oropharyngeal squamous cell carcinomas (OSCCs), being detected in 50% to 80% of tumors by DNA in situ hybridization (ISH) and/or polymerase chain reaction. However, these tests do not assess viral transcription. Many consider E6/E7 messenger ribonucleic acid (mRNA) the best indicator of HPV status, but it has not been detected in situ in OSCC. We constructed tissue microarrays (TMAs) from a cohort of OSCC for which p16 immunohistochemistry and HPV DNA ISH were previously performed on whole sections. We utilized a novel, chromogenic RNA ISH assay called RNAscope to detect E6/E7 mRNA of HPV-16 and other high-risk types on these TMAs. RNA ISH results were obtained for 196 of 211 TMA cases, of which 153 (78.1%) were positive. p16 immunohistochemistry and HPV DNA ISH were positive in 79.0% and 62.4% of cases, respectively. Concordance between RNA and p16, DNA and p16, and RNA and DNA were 96.4%, 78.7%, and 83.5%, respectively. Only 7 cases (3.6%) were discrepant between RNA ISH and p16. In univariate analysis, all 3 tests correlated with better overall survival (OS), disease-specific survival (DSS), and disease-free survival (DFS) (all P<0.001). In multivariate analysis, OS correlated significantly with RNA (hazard ratio=0.39, P=0.001), DNA (0.53, P=0.03), and p16 (0.30, P<0.001), but DSS and DFS correlated significantly only with p16 (DSS: 0.36, P=0.006; DFS: 0.42, P=0.016). RNA ISH is more sensitive than DNA ISH in detecting HPV in OSCC, and it correlates strongly with p16. Although both tests were comparable, p16 more strongly stratified patient outcomes.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological
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| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1532-0979
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
35
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1343-50
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| pubmed:meshHeading |
pubmed-meshheading:21836494-Adult,
pubmed-meshheading:21836494-Aged,
pubmed-meshheading:21836494-Aged, 80 and over,
pubmed-meshheading:21836494-Carcinoma, Squamous Cell,
pubmed-meshheading:21836494-Cyclin-Dependent Kinase Inhibitor p16,
pubmed-meshheading:21836494-Disease-Free Survival,
pubmed-meshheading:21836494-Female,
pubmed-meshheading:21836494-Humans,
pubmed-meshheading:21836494-Immunohistochemistry,
pubmed-meshheading:21836494-In Situ Hybridization,
pubmed-meshheading:21836494-Kaplan-Meier Estimate,
pubmed-meshheading:21836494-Male,
pubmed-meshheading:21836494-Middle Aged,
pubmed-meshheading:21836494-Missouri,
pubmed-meshheading:21836494-Oncogene Proteins, Viral,
pubmed-meshheading:21836494-Oropharyngeal Neoplasms,
pubmed-meshheading:21836494-Papillomaviridae,
pubmed-meshheading:21836494-Predictive Value of Tests,
pubmed-meshheading:21836494-Prognosis,
pubmed-meshheading:21836494-Proportional Hazards Models,
pubmed-meshheading:21836494-RNA, Messenger,
pubmed-meshheading:21836494-RNA, Viral,
pubmed-meshheading:21836494-Risk Assessment,
pubmed-meshheading:21836494-Risk Factors,
pubmed-meshheading:21836494-Survival Rate,
pubmed-meshheading:21836494-Time Factors,
pubmed-meshheading:21836494-Tissue Array Analysis,
pubmed-meshheading:21836494-Tumor Markers, Biological
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| pubmed:year |
2011
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| pubmed:articleTitle |
High-risk human papillomavirus E6/E7 mRNA detection by a novel in situ hybridization assay strongly correlates with p16 expression and patient outcomes in oropharyngeal squamous cell carcinoma.
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| pubmed:affiliation |
Department of Pathology and Immunology, Washington University, 660 S. Euclid Ave., St. Louis, MO 63110, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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