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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-5-24
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pubmed:abstractText |
K2 neutral strain Saccharomyces cerevisiae USM12 was identified and characterized. This strain carried an M double-stranded RNA (dsRNA) genome encoding for resistance to K2 toxin. The M dsRNA was larger than the K2 killer yeast M dsRNA and homoduplex analysis of denatured and reannealed K2 neurtal M dsRNA revealed an inverted duplication. Heteroduplex analysis showed that two thirds of the K2 M genome had homology with the K2 neutral M genome. Hybridization showed that the USM12 M dsRNA had significant homology with the K2 M dsRNA. Protein profiles of extracellular proteins from USM12 and a cured strain indicated that USM12 did not secrete any toxin. This is the first time that a K2 neutral yeast strain has been characterized.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0749-503X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
159-69
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2183523-Cycloheximide,
pubmed-meshheading:2183523-Drug Resistance, Microbial,
pubmed-meshheading:2183523-Genes, Fungal,
pubmed-meshheading:2183523-Killer Factors, Yeast,
pubmed-meshheading:2183523-Microscopy, Electron,
pubmed-meshheading:2183523-Mycotoxins,
pubmed-meshheading:2183523-Nucleic Acid Heteroduplexes,
pubmed-meshheading:2183523-Nucleic Acid Hybridization,
pubmed-meshheading:2183523-RNA, Double-Stranded,
pubmed-meshheading:2183523-Saccharomyces cerevisiae,
pubmed-meshheading:2183523-Saccharomyces cerevisiae Proteins
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pubmed:articleTitle |
A K2 neutral Saccharomyces cerevisiae strain contains a variant K2 M genome.
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pubmed:affiliation |
Department of Microbiology, University of Stellenbosch, South Africa.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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