Linked Life Data

2183467

Source:http://linkedlifedata.com/resource/pubmed/id/2183467

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-5-23
pubmed:abstractText
The complete nucleotide sequences and translations of major open reading frames (ORF) of two distinct, infectious, proviral molecular clones (106 and 127) of the bovine immunodeficiency-like virus (BIV), obtained from a single virus isolation, were determined and compared. The genomes of BIV 127 and 106 are 8482 and 8391 nucleotides (nt), respectively, in the form predicted for the viral RNA. The structural organization of the genomes of BIV 127 and 106 are identical to one another and most similar to that of the lentivirus subfamily of retroviruses. In addition to gag, pol, and env genes, the BIV genome contains five short ORFs between and overlapping pol and env in the "central region," a hallmark of the lentiviruses which is believed to play an important role in their pathogenesis. Three of the short ORFs in the central region of BIV have been identified by location and structural similarity to the nonstructural/regulatory genes (vif, tat, and rev) of other lentiviruses; we also discovered two unique ORFs, termed W and Y, which may serve as exons for novel genes. BIV does not have the nef gene found in primate lentivirus genomes. The proviral LTR of BIV 127 is 589 nt, contains regulatory signals for initiation, enhancement, and termination of viral transcription, and has sequences related to the Sp1 and NF-kappa B binding sites. A major deletion (87 nt) in the env gene and 2 minor deletions (2 nt each) in the R regions of the LTRs account for the smaller size of clone 106. Numerous point mutations were also present; some caused coding substitutions that were most prevalent in the env encoding ORF. These data suggest that, within a single virus isolate, BIV displays extensive genomic variation. These infectious clones of BIV represent well-defined tools with which to analyze the function of the various ORFs and to dissect the molecular mechanisms of replication and pathogenesis.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0042-6822
pubmed:author
pubmed:issnType
Print
pubmed:volume
175
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
391-409
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:2183467-Amino Acid Sequence, pubmed-meshheading:2183467-Animals, pubmed-meshheading:2183467-Base Sequence, pubmed-meshheading:2183467-Cattle, pubmed-meshheading:2183467-Cells, Cultured, pubmed-meshheading:2183467-Cloning, Molecular, pubmed-meshheading:2183467-DNA, Viral, pubmed-meshheading:2183467-Gene Products, env, pubmed-meshheading:2183467-Gene Products, gag, pubmed-meshheading:2183467-Gene Products, pol, pubmed-meshheading:2183467-Genes, Viral, pubmed-meshheading:2183467-Genetic Variation, pubmed-meshheading:2183467-Molecular Sequence Data, pubmed-meshheading:2183467-Molecular Weight, pubmed-meshheading:2183467-Phylogeny, pubmed-meshheading:2183467-Proviruses, pubmed-meshheading:2183467-RNA, Viral, pubmed-meshheading:2183467-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:2183467-Retroviridae, pubmed-meshheading:2183467-Retroviridae Proteins, pubmed-meshheading:2183467-Sequence Homology, Nucleic Acid, pubmed-meshheading:2183467-Transcription, Genetic
pubmed:year
1990
pubmed:articleTitle
Nucleotide sequence and genome organization of biologically active proviruses of the bovine immunodeficiency-like virus.
pubmed:affiliation
Laboratory of Cell and Molecular Structure, NCl-Frederick Cancer Research Facility, Maryland 21701.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.