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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4952
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pubmed:dateCreated |
1990-5-17
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pubmed:abstractText |
The product of the c-abl proto-oncogene (c-Abl) is phosphorylated on three sites during interphase and seven additional sites during mitosis. Two interphase and all mitotic c-Abl sites are phosphorylated by cdc2 kinase isolated from either interphase or mitotic cells, with the mitotic cdc2 having an 11-fold higher activity. Inhibition of phosphatases with okadaic acid in interphase cells leads to the phosphorylation of c-Abl mitotic sites, indicating that those sites are preferentially dephosphorylated during interphase. The differential phosphorylation of c-Abl in the cell cycle is therefore determined by an equilibrium between cdc2 kinase and protein phosphatase activities. Treatment of interphase cells with okadaic acid leads to a rounded morphology similar to that observed during mitosis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
248
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
217-20
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:2183353-Amino Acid Sequence,
pubmed-meshheading:2183353-Animals,
pubmed-meshheading:2183353-CDC2 Protein Kinase,
pubmed-meshheading:2183353-Cell Cycle,
pubmed-meshheading:2183353-Cells, Cultured,
pubmed-meshheading:2183353-Ethers, Cyclic,
pubmed-meshheading:2183353-Interphase,
pubmed-meshheading:2183353-Mice,
pubmed-meshheading:2183353-Mice, Inbred Strains,
pubmed-meshheading:2183353-Molecular Sequence Data,
pubmed-meshheading:2183353-Molecular Weight,
pubmed-meshheading:2183353-Okadaic Acid,
pubmed-meshheading:2183353-Peptide Fragments,
pubmed-meshheading:2183353-Peptide Mapping,
pubmed-meshheading:2183353-Phosphoproteins,
pubmed-meshheading:2183353-Phosphorylation,
pubmed-meshheading:2183353-Protein-Tyrosine Kinases,
pubmed-meshheading:2183353-Proto-Oncogene Proteins,
pubmed-meshheading:2183353-Proto-Oncogene Proteins c-abl
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pubmed:year |
1990
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pubmed:articleTitle |
Differential phosphorylation of c-Abl in cell cycle determined by cdc2 kinase and phosphatase activity.
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pubmed:affiliation |
Department of Biology, University of California, San Diego, La Jolla 92093.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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