Linked Life Data

2182827

Source:http://linkedlifedata.com/resource/pubmed/id/2182827

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1990-5-14
pubmed:abstractText
A passive hemagglutination assay (PHA) for serum human immunodeficiency virus type 1 (HIV-1) antibody screening was developed using aldehyde-stabilized human erythrocytes coated with purified HIV-1 antigens. The assay is simple to perform, and the components are inexpensive. In preliminary testing of a panel of 490 confirmed HIV-1 seropositive specimens, all tested positive by the HIV-1 PHA assay. By testing seroconversion specimens and dilution panels, the assay demonstrated a sensitivity equivalent to a recombinant protein-based HIV-1 enzyme immunoassay (EIA). In-house evaluation of 2,557 HIV-1 prescreened specimens from South African blood bank donors revealed initial and repeat reactive rates of 0.2 and 0.04%, respectively. Field testing of the HIV-1 antibody PHA at two sites gave performance of 100% sensitivity (400 seroconfirmed samples) and 0.55% initial/0.28% repeat reactive rates in the testing of 3,983 negative samples. The performance, low cost, and ease of use make the HIV-1 antibody PHA test a prime candidate for HIV-1 antibody screening in regions of the world where more sophisticated technology is inappropriate.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0894-9255
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
540-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
RETROCELL HIV-1 passive hemagglutination assay for HIV-1 antibody screening.
pubmed:affiliation
Abbott Laboratories, Diagnostics Division, North Chicago, Illinois 60064.
pubmed:publicationType
Journal Article, Comparative Study