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rdf:type |
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lifeskim:mentions |
umls-concept:C0008562,
umls-concept:C0013935,
umls-concept:C0025663,
umls-concept:C0032105,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0036563,
umls-concept:C0068486,
umls-concept:C0185125,
umls-concept:C0201734,
umls-concept:C0214131,
umls-concept:C0439831,
umls-concept:C0521115,
umls-concept:C0680730,
umls-concept:C0973447,
umls-concept:C1148554,
umls-concept:C1568042
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pubmed:issue |
6
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pubmed:dateCreated |
2011-8-10
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pubmed:abstractText |
A rapid and specific HPLC method has been developed and validated for the simultaneous determination of liensinine (CAS 2586-96-1), isoliensinine (CAS 6817-41-0) and neferine (CAS 2292-16-2) in rat plasma. The sample was prepared by a liquid-liquid extraction with diethyl ether and the recovery was above 80% from the plasma for the three compounds. Chromatographic separation was achieved with a Hypersil BDS C18 column (4.0 mm x 250 mm, particle size 5 microm). A mobile phase consisting of methanol: 0.2 M KH2PO4:0.2 M NaOH:triethylamine (71:17:12:0.002, v/v/v/v, pH 9.2-9.3) was slowly delivered at 0.8 ml/min in isocratic mode with a detection wavelength of 282 nm. The linearity of calibration curves were good (r > 0.999) in the concentration range of 0.031-2.00 microg/ ml. The lower limit of quantification can reach 0.03 microg/ml for the three compounds. The intra-day and inter-day variations estimated with QC samples were less than 8% for the three tested concentration levels. This developed method was applied in the plasma pharmacokinetic study of total bisbenzylisoquinoline alkaloids (TAL) of the lotus flower (Lian Zi Xin) following a single oral and intravenous administration of TAL in rats.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0004-4172
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
347-52
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pubmed:meshHeading |
pubmed-meshheading:21827045-Administration, Oral,
pubmed-meshheading:21827045-Animals,
pubmed-meshheading:21827045-Area Under Curve,
pubmed-meshheading:21827045-Benzylisoquinolines,
pubmed-meshheading:21827045-Calibration,
pubmed-meshheading:21827045-Chromatography, High Pressure Liquid,
pubmed-meshheading:21827045-Female,
pubmed-meshheading:21827045-Half-Life,
pubmed-meshheading:21827045-Injections, Intravenous,
pubmed-meshheading:21827045-Isoquinolines,
pubmed-meshheading:21827045-Male,
pubmed-meshheading:21827045-Nelumbo,
pubmed-meshheading:21827045-Phenols,
pubmed-meshheading:21827045-Rats,
pubmed-meshheading:21827045-Rats, Wistar,
pubmed-meshheading:21827045-Reference Standards,
pubmed-meshheading:21827045-Reproducibility of Results,
pubmed-meshheading:21827045-Seeds,
pubmed-meshheading:21827045-Solutions,
pubmed-meshheading:21827045-Spectrophotometry, Ultraviolet
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pubmed:year |
2011
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pubmed:articleTitle |
Simultaneous determination of liensinine, isoliensinine and neferine from seed embryo of Nelumbo nucifera Gaertn. in rat plasma by a rapid HPLC method and its application to a pharmacokinetic study.
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pubmed:affiliation |
WuXi AppTec Co., Ltd, Wuxi, P R China.
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pubmed:publicationType |
Journal Article
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