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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1990-5-17
|
| pubmed:abstractText |
Total mitochondrial aspartate aminotransferase (EC 2.6.1.1), the sum of apo- and holo-mitochondrial aspartate aminotransferase activity in human serum, was measured by using a proteolytic method: inactivation of cytosolic aspartate aminotransferase with cytosolic aspartate aminotransferase-inactivating protease 401 from Streptomyces violaceochromogenes. Cytosolic aspartate aminotransferase is completely inactivated, and apo-mitochondrial aspartate aminotransferase is completely activated by pyridoxal 5'-phosphate within 5 min. Results by the proposed method correlated well with those by an immunochemical method (r = 0.994, n = 145) and showed excellent inhibitory activity of the protease for holo- and apo-cytosolic aspartate aminotransferase up to 5000 U/L and activation of mitochondrial apo-aspartate aminotransferase up to 2000 U/L in the presence of 100 mumol of pyridoxal 5'-phosphate per liter. Within-run Cvs were good (1.13-7.49%). Mean values for total mitochondrial aspartate aminotransferase and apo-mitochondrial aspartate aminotransferase activities in serum of the healthy subjects were 4.8 (SD 0.9) and 1.8 (SD 0.8) U/L, respectively (n = 154). Various common interferents tested did not affect this assay.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0009-9147
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
36
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
687-9
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2182221-Aspartate Aminotransferases,
pubmed-meshheading:2182221-Autoanalysis,
pubmed-meshheading:2182221-Cytosol,
pubmed-meshheading:2182221-Enzyme Activation,
pubmed-meshheading:2182221-Humans,
pubmed-meshheading:2182221-Hydrogen-Ion Concentration,
pubmed-meshheading:2182221-Indicators and Reagents,
pubmed-meshheading:2182221-Mitochondria, Liver,
pubmed-meshheading:2182221-Peptide Hydrolases,
pubmed-meshheading:2182221-Pyridoxal Phosphate,
pubmed-meshheading:2182221-Streptomyces
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
New automated measurement of mitochondrial aspartate aminotransferase with use of protease 401.
|
| pubmed:affiliation |
Department of Laboratory Medicine, Kumamoto University Medical School, Japan.
|
| pubmed:publicationType |
Journal Article
|