Linked Life Data

21818803

Source:http://linkedlifedata.com/resource/pubmed/id/21818803

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2011-8-5
pubmed:abstractText
In this study, a fast and reliable method based on an ultra-high-pressure liquid chromatography coupled with photodiode-array detection (PDA) and a linear ion trap high-resolution mass spectrometer (LTQ-Orbitrap XL) has been developed for the identification of bioactive constituents in the whole plant of Sarcandra glabra and its related four preparations. By accurate mass measurements within 5?ppm error for each molecular ion and subsequent fragment ions in routine analysis, 50 compounds, including organic acids, caffeoyl derivatives, flavonoids, coumarins and terpenoids, were identified or tentatively characterized. Among them, 6,7,8-trihydroxycoumarin-O-rhamnopyranoside (17), (2R)-naringenin-6-C-B-D-glucopyranosyl-(6?1)-apiose (25) and (2S)-naringenin-6-C-B-D-glucopyranosyl-(6?1)-apiose (27) were tentatively identified as new compounds. Besides, 21 of these compounds were coexisting in four preparations of Sarcandra glabra. Fragmentation behaviors of the four major categories of compounds were also investigated. This established UPLC-PDA/ESI-MS(n) method was reliable and effective for the separation and identification of the major constituents and would be the basis for quality control of Sarcandra glabra and its related preparations.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
1097-0231
pubmed:author
pubmed:copyrightInfo
Copyright © 2011 John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2439-47
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Chemical profiling of bioactive constituents in Sarcandra glabra and its preparations using ultra-high-pressure liquid chromatography coupled with LTQ Orbitrap mass spectrometry.
pubmed:affiliation
Second Affiliated Hospital, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't