Source:http://linkedlifedata.com/resource/pubmed/id/21807912
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
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pubmed:dateCreated |
2011-9-22
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pubmed:abstractText |
We have previously reported that compromised interleukin 17A (IL-17A) production in the lungs increased susceptibility to infection with the invasive fungal pathogen Aspergillus fumigatus. Here we have shown that culturing lung cells from A. fumigatus-challenged mice ex vivo demonstrated Dectin-1-dependent IL-17A production. In this system, neutralization of IL-23 but not IL-6, IL-1?, or IL-18 resulted in attenuated IL-17A production. Il23 mRNA expression was found to be lower in lung cells from A. fumigatus-challenged Dectin-1-deficient mice, whereas bone marrow-derived dendritic cells from Dectin-1-deficient mice failed to produce IL-23 in response to A. fumigatus in vitro. Addition of recombinant IL-23 augmented IL-17A production by wild-type (WT) and Dectin-1-deficient lung cells, although the addition of IL-6 or IL-1? did not augment the effect of IL-23. Intracellular cytokine staining of lung cells revealed lower levels of CD11b(+) IL-17A(+) and Ly-6G(+) IL-17A(+) cells in A. fumigatus-challenged Dectin-1-deficient mice. Ly-6G(+) neutrophils purified from the lungs of A. fumigatus-challenged Dectin-1-deficient mice displayed lower Il17a mRNA expression but surprisingly had intact Rorc and Rora mRNA expression. We further demonstrated that Ly-6G(+) neutrophils required the presence of myeloid cells for IL-17A production. Finally, upon in vitro stimulation with A. fumigatus, thioglycolate-elicited peritoneal neutrophils were positive for intracellular IL-17A expression and produced IL-17A in a Dectin-1- and IL-23-dependent manner. In summary, Dectin-1-dependent IL-17A production in the lungs during invasive fungal infection is mediated in part by CD11b(+) Ly-6G(+) neutrophils in an IL-23-dependent manner.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-17,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-23,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/dectin 1
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1098-5522
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pubmed:author |
pubmed-author:BrownGordon DGD,
pubmed-author:ChaplinDavid DDD,
pubmed-author:DeshaneJessyJ,
pubmed-author:DunawayChad WCW,
pubmed-author:GessnerMelissa AMA,
pubmed-author:HornDawnD,
pubmed-author:LillyLauren MLM,
pubmed-author:MetzAllison EAE,
pubmed-author:NelsonMichael PMP,
pubmed-author:SteeleChadC,
pubmed-author:WeaverCasey TCT,
pubmed-author:WernerJessica LJL
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pubmed:issnType |
Electronic
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3966-77
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pubmed:meshHeading |
pubmed-meshheading:21807912-Animals,
pubmed-meshheading:21807912-Aspergillus fumigatus,
pubmed-meshheading:21807912-Cells, Cultured,
pubmed-meshheading:21807912-Interleukin-17,
pubmed-meshheading:21807912-Interleukin-23,
pubmed-meshheading:21807912-Lung,
pubmed-meshheading:21807912-Membrane Proteins,
pubmed-meshheading:21807912-Mice,
pubmed-meshheading:21807912-Mice, Inbred C57BL,
pubmed-meshheading:21807912-Nerve Tissue Proteins,
pubmed-meshheading:21807912-Neutrophils,
pubmed-meshheading:21807912-Pulmonary Aspergillosis
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pubmed:year |
2011
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pubmed:articleTitle |
Neutrophils produce interleukin 17A (IL-17A) in a dectin-1- and IL-23-dependent manner during invasive fungal infection.
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pubmed:affiliation |
Department of Medicine, School of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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