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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2011-9-22
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pubmed:abstractText |
The creation of subunit vaccines to prevent malaria infection has been hampered by the intrinsically weak immunogenicity of the recombinant antigens. We have developed a novel strategy to increase immune responses by creating genetic fusion proteins to target specific antigen-presenting cells (APCs). The fusion complex was composed of three physically linked molecular entities: (i) a vaccine antigen, (ii) a multimeric ?-helical coiled-coil core, and (iii) an APC-targeting ligand linked to the core via a flexible linker. The vaccine efficacy of the tricomponent complex was evaluated using an ookinete surface protein of Plasmodium vivax, Pvs25, and merozoite surface protein-1 of Plasmodium yoelii. Immunization of mice with the tricomponent complex induced a robust antibody response and conferred substantial levels of P. vivax transmission blockade as evaluated by a membrane feed assay, as well as protection from lethal P. yoelii infection. The observed effect was strongly dependent on the presence of all three components physically integrated as a fusion complex. This system, designated the tricomponent immunopotentiating system (TIPS), onto which any recombinant protein antigens or nonproteinaceous substances could be loaded, may be a promising strategy for devising subunit vaccines or adjuvants against various infectious diseases, including malaria.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Protozoan,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Malaria Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Merozoite Surface Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Pvs25 protein, P vivax,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/cartilage matrix protein
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1098-5522
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
79
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4260-75
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pubmed:meshHeading |
pubmed-meshheading:21807905-Amino Acid Sequence,
pubmed-meshheading:21807905-Animals,
pubmed-meshheading:21807905-Antigen-Presenting Cells,
pubmed-meshheading:21807905-Antigens, Protozoan,
pubmed-meshheading:21807905-Antigens, Surface,
pubmed-meshheading:21807905-B-Lymphocytes,
pubmed-meshheading:21807905-Base Sequence,
pubmed-meshheading:21807905-Drug Design,
pubmed-meshheading:21807905-Extracellular Matrix Proteins,
pubmed-meshheading:21807905-Female,
pubmed-meshheading:21807905-Glycoproteins,
pubmed-meshheading:21807905-Ligands,
pubmed-meshheading:21807905-Lymphocyte Activation,
pubmed-meshheading:21807905-Malaria,
pubmed-meshheading:21807905-Malaria, Vivax,
pubmed-meshheading:21807905-Malaria Vaccines,
pubmed-meshheading:21807905-Merozoite Surface Protein 1,
pubmed-meshheading:21807905-Mice,
pubmed-meshheading:21807905-Mice, Inbred BALB C,
pubmed-meshheading:21807905-Mice, Inbred C57BL,
pubmed-meshheading:21807905-Molecular Sequence Data,
pubmed-meshheading:21807905-Plasmodium vivax,
pubmed-meshheading:21807905-Plasmodium yoelii,
pubmed-meshheading:21807905-Recombinant Fusion Proteins,
pubmed-meshheading:21807905-Vaccines, Subunit,
pubmed-meshheading:21807905-Vaccines, Synthetic
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pubmed:year |
2011
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pubmed:articleTitle |
Tricomponent immunopotentiating system as a novel molecular design strategy for malaria vaccine development.
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pubmed:affiliation |
Molecular Microbiology Group, Tropical Biosphere Research Center, COMB, University of the Ryukyus, Okinawa Japan. tarakawa@comb.u-ryukyu.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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