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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1990-4-26
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pubmed:abstractText |
The interaction of azole antifungal antibiotics with purified Candida albicans cytochrome P-450-dependent 14 alpha-sterol demethylase (P-450DM) was measured spectrophotometrically and by inhibition of enzyme activity. Ketoconazole and ICI 153066 (a triazole derivative) formed low-spin complexes with the ferric cytochrome and induced type II difference spectra. These spectra are indicative of an interaction between the azole moiety and the sixth co-ordination position of P-450DM haem. Both azoles inhibited the binding of CO to the sodium dithionite-reduced ferrous cytochrome, and inhibited reconstituted P-450DM activity by binding to the cytochrome with a one-to-one stoichiometry. Similarly, total inhibition of enzyme activity occurred when equimolar amounts of clotrimazole, miconazole or fluconazole were added to reconstituted P-450DM. These results correlated with the inhibition of P-450DM in broken cell preparations, confirming that all five azoles are potent inhibitors of ergosterol biosynthesis in C. albicans.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-14209971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-2556999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-2644625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-2669735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-270726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3069196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3111296,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3322742,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3323450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3521608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3522841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3543000,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3545213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3771545,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-3916772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-6363414,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-6414474,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-6420412,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-6760419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2180400-7061465
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antifungal Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Azoles,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon Monoxide,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Ferric Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/ICI 153066,
http://linkedlifedata.com/resource/pubmed/chemical/Ketoconazole,
http://linkedlifedata.com/resource/pubmed/chemical/Mevalonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/Sterol 14-Demethylase,
http://linkedlifedata.com/resource/pubmed/chemical/Triazoles
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
266
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
475-80
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:2180400-Antifungal Agents,
pubmed-meshheading:2180400-Azoles,
pubmed-meshheading:2180400-Candida albicans,
pubmed-meshheading:2180400-Carbon Monoxide,
pubmed-meshheading:2180400-Cytochrome P-450 Enzyme System,
pubmed-meshheading:2180400-Ferric Compounds,
pubmed-meshheading:2180400-Ketoconazole,
pubmed-meshheading:2180400-Mevalonic Acid,
pubmed-meshheading:2180400-Oxidoreductases,
pubmed-meshheading:2180400-Spectrum Analysis,
pubmed-meshheading:2180400-Sterol 14-Demethylase,
pubmed-meshheading:2180400-Triazoles
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pubmed:year |
1990
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pubmed:articleTitle |
Interaction of azole antifungal antibiotics with cytochrome P-450-dependent 14 alpha-sterol demethylase purified from Candida albicans.
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pubmed:affiliation |
Department of Microbiology, University of Leeds, U.K.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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