Linked Life Data

21803436

Source:http://linkedlifedata.com/resource/pubmed/id/21803436

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-8-15
pubmed:abstractText
Due to the fact that probiotic cells need to be alive when they are consumed, culture-based analysis (plate count) is critical in ascertaining the quality (numbers of viable cells) of probiotic products. Since probiotic cells are typically stressed, due to various factors related to their production, processing and formulation, the standard methodology for total plate counts tends to underestimate the cell numbers of these products. Furthermore, products such as microencapsulated cultures require modifications in the release and sampling procedure in order to correctly estimate viable counts. This review examines the enumeration of probiotic bacteria in the following commercial products: powders, microencapsulated cultures, frozen concentrates, capsules, foods and beverages. The parameters which are specifically examined include: sample preparation (rehydration, thawing), dilutions (homogenization, media) and plating (media, incubation) procedures. Recommendations are provided for each of these analytical steps to improve the accuracy of the analysis. Although the recommendations specifically target the analysis of probiotics, many will apply to the analysis of commercial lactic starter cultures used in food fermentations as well.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1879-3460
pubmed:author
pubmed:copyrightInfo
Crown Copyright © 2011. Published by Elsevier B.V. All rights reserved.
pubmed:issnType
Electronic
pubmed:day
3
pubmed:volume
149
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
185-93
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Recommendations for the viability assessment of probiotics as concentrated cultures and in food matrices.
pubmed:affiliation
Agriculture and Agri-food Canada, Food Research and Development Centre, 3600 Casavant Blvd, St. Hyacinthe, QC Canada J2S 8E3. claude.champagne@agr.gc.ca
pubmed:publicationType
Journal Article, Review