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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0044602,
umls-concept:C0185117,
umls-concept:C0220847,
umls-concept:C0598312,
umls-concept:C0677626,
umls-concept:C0851285,
umls-concept:C1101610,
umls-concept:C1314939,
umls-concept:C1515926,
umls-concept:C1704259,
umls-concept:C1705987,
umls-concept:C1979963,
umls-concept:C2003903,
umls-concept:C2911684
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pubmed:issue |
1
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pubmed:dateCreated |
2011-8-22
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pubmed:abstractText |
The aim of this study was to investigate the role of microRNA (miRNA) on hepatitis C virus (HCV) replication in hepatoma cells. Using miRNA array analysis, miR-192/miR-215, miR-194, miR-320, and miR-491 were identified as miRNAs whose expression levels were altered by HCV infection. Among them, miR-192/miR-215 and miR-491 were capable of enhancing replication of the HCV replicon as well as HCV itself. HCV IRES activity or cell proliferation was not increased by forced expression of miR-192/miR-215 or miR-491. Investigation of signaling pathways revealed that miR-491 specifically suppressed the phosphoinositol-3 (PI3) kinase/Akt pathway. Under inhibition of PI3 kinase by LY294002, the suppressive effect of miR-491 on HCV replication was abolished, indicating that suppression of HCV replication by miR-491 was dependent on the PI3 kinase/Akt pathway. miRNAs altered by HCV infection would then affect HCV replication, which implies a complicated mechanism for regulating HCV replication. HCV-induced miRNA may be involved in changes in cellular properties including hepatocarcinogenesis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1090-2104
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pubmed:author |
pubmed-author:HayashiNorioN,
pubmed-author:HikitaHayatoH,
pubmed-author:HiramatsuNaokiN,
pubmed-author:HosuiAtsushiA,
pubmed-author:IshidaHisashiH,
pubmed-author:KantoTatsuyaT,
pubmed-author:KodamaTakahiroT,
pubmed-author:NawaTakatoshiT,
pubmed-author:ShimizuSatoshiS,
pubmed-author:TakeharaTetsuoT,
pubmed-author:TatsumiTomohideT
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pubmed:copyrightInfo |
Copyright © 2011 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
19
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pubmed:volume |
412
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
92-7
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pubmed:meshHeading |
pubmed-meshheading:21802413-Carcinoma, Hepatocellular,
pubmed-meshheading:21802413-Cell Line, Tumor,
pubmed-meshheading:21802413-Hepacivirus,
pubmed-meshheading:21802413-Hepatitis C,
pubmed-meshheading:21802413-Humans,
pubmed-meshheading:21802413-Liver Neoplasms,
pubmed-meshheading:21802413-MicroRNAs,
pubmed-meshheading:21802413-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:21802413-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:21802413-Virus Replication
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pubmed:year |
2011
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pubmed:articleTitle |
Alterations in microRNA expression profile in HCV-infected hepatoma cells: involvement of miR-491 in regulation of HCV replication via the PI3 kinase/Akt pathway.
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pubmed:affiliation |
Department of Gastroenterology and Hepatology, Osaka University Graduate School of Medicine, 2-2, Yamadaoka, Suita 565-0871, Japan.
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pubmed:publicationType |
Journal Article
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