Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1990-4-25
|
| pubmed:abstractText |
The Abbott TDx fluorescence polarization immunoassay (FPIA) procedure for measuring cyclosporine A (CsA) was evaluated and compared with the Sandoz polyclonal radioimmunoassay (CsA RIA kit) method. This drug assay was evaluated for precision, calibration, stability, and accuracy. Within-run precision studies utilizing 25 replicate analyses of the three control preparations (containing CsA in the 60-800 ng/ml range) resulted in coefficients of variation (CV) ranging from 1.0 to 9.1%. The CVs of between-run precision determined by assaying the same control drug levels for five consecutive working days ranged from 3.9 to 4.6%. Calibration curve stability was assessed by examining the drift in control values over a 2-week period. Maximum plasma ranged from 82.6 to 108.2%. Four hundred plasma samples were obtained from 30 heart-transplant patients during the first 6 months of CsA therapy and each sample was analyzed simultaneously by TDx and RIA. Linear regression analysis of the results obtained for each patient (x = RIA, y = FPIA) revealed the following mean values: r = 0.87, (CV = 13.7%), slope = 1.47 (CV = 39.2%). Moreover, the concentration of CsA was determined in 35 patient samples both by TDx and high-performance liquid chromatography (HPLC). FPIA results up to 12 times higher than HPLC results have been noted.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0163-4356
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
182-6
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2180138-Chromatography, High Pressure Liquid,
pubmed-meshheading:2180138-Cyclosporins,
pubmed-meshheading:2180138-Fluorescence Polarization,
pubmed-meshheading:2180138-Fluorescent Antibody Technique,
pubmed-meshheading:2180138-Heart Transplantation,
pubmed-meshheading:2180138-Humans,
pubmed-meshheading:2180138-Middle Aged,
pubmed-meshheading:2180138-Radioimmunoassay
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Measurement of cyclosporine in plasma of cardiac allograft recipients by fluorescence polarization immunoassay.
|
| pubmed:affiliation |
Department of Pharmacology, IRCCS, Policlinico S. Matteo, Pavia, Italy.
|
| pubmed:publicationType |
Journal Article
|