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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1990-4-20
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pubmed:abstractText |
Our aim was to determine whether oestrogen administered to ovariectomized rats in a manner which stimulates the release of LHRH also stimulates the synthesis of LHRH mRNA. Adult female Wistar rats were ovariectomized under halothane anaesthesia between 09.00 and 11.00 h of dioestrus, immediately injected with either oil or 10 micrograms oestradiol benzoate (OB) and then killed by cervical dislocation between 16.00 and 17.00 h of the next day (presumptive pro-oestrus). In-situ hybridization carried out with a 30 mer, 32P-labelled, oligonucleotide probe complementary to LHRH mRNA showed that the concentrations of LHRH mRNA in perikarya in the medial preoptic area, diagonal band and medial septum were significantly greater in OB-compared with oil-treated rats. Plasma LH concentrations were significantly increased in three out of four of the OB-treated rats. These results show for the first time that as well as stimulating LHRH and LH release, oestrogen positive feedback also stimulates the synthesis of LHRH mRNA.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-0795
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
124
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
285-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:2179457-Animals,
pubmed-meshheading:2179457-Estradiol,
pubmed-meshheading:2179457-Feedback,
pubmed-meshheading:2179457-Female,
pubmed-meshheading:2179457-Gonadotropin-Releasing Hormone,
pubmed-meshheading:2179457-Hypothalamus,
pubmed-meshheading:2179457-Hypothalamus, Anterior,
pubmed-meshheading:2179457-Luteinizing Hormone,
pubmed-meshheading:2179457-Neurons,
pubmed-meshheading:2179457-Ovariectomy,
pubmed-meshheading:2179457-RNA, Messenger,
pubmed-meshheading:2179457-Rats,
pubmed-meshheading:2179457-Rats, Inbred Strains
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pubmed:year |
1990
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pubmed:articleTitle |
Oestrogen positive feedback stimulates the synthesis of LHRH mRNA in neurones of the rostral diencephalon of the rat.
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pubmed:affiliation |
MRC Brain Metabolism Unit, University Department of Pharmacology, Edinburgh.
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pubmed:publicationType |
Journal Article
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