Linked Life Data

21791952

Source:http://linkedlifedata.com/resource/pubmed/id/21791952

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2011-7-27
pubmed:abstractText
DNA methylation in Streptomyces griseus IFO 13350 was analyzed by high-performance liquid chromatographic analysis and bisulfite-based analysis to reveal two methylation sites, 5'-GC5mCGGC-3' and 5'-GAG5mCTC-3'. The methylation was reconstituted in Escherichia coli by simultaneous expression of S. griseus SGR4675 and S. achromogenes M.SacI. The E. coli cells produced plasmids that mimicked the methylation profile of S. griseus DNA, which was readily introduced into S. griseus. The results of this study raise the possibility of a promising approach to establish efficient transformation in several streptomycetes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1738-8872
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
675-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Improvement of transformation efficiency by strategic circumvention of restriction barriers in Streptomyces griseus.
pubmed:affiliation
Chemical Biology Department, Advanced Science Institute, RIKEN, Saitama 351-0198, Japan. hirokap@xpost.plala.or.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't