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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-3-20
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pubmed:abstractText |
The effect of thyrotropin (TSH) on cyclic AMP accumulation, phosphatidylinositol bisphosphate (PIP2) hydrolysis and [Ca2+]i rise has been studied in CHO cells stably transfected with human TSH receptor (hTSHR) cDNA. In human thyroid slices, TSH activates these two intracellular cascades with a higher affinity for the adenylate cyclase activation (from 0.1 to 1 mU/ml TSH) than for phospholipase C activation (from 1 to 10 mU/ml TSH). The CHO cells transfected with the recently cloned cDNA of human TSH receptor respond in the same way to TSH. They respond between 0.1 and 1 mU/ml TSH for cyclic AMP accumulation and between 1 and 10 mU/ml TSH for inositol monophosphate (IP1) increase. In these same cells, TSH 10 mU/ml, but not forskolin (10 microM), or dibutyryl cyclic AMP (100 microM), clearly enhances intracellular calcium concentration [( Ca2+]i). Our results demonstrate unequivocally that a single transcription unit has the potential to encode receptor molecules coupled to both cascades.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Bucladesine,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 4,5-Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thyrotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Thyrotropin,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0303-7207
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
12
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
R1-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:2178105-Adenylate Cyclase,
pubmed-meshheading:2178105-Animals,
pubmed-meshheading:2178105-Bucladesine,
pubmed-meshheading:2178105-Calcium,
pubmed-meshheading:2178105-Cell Line,
pubmed-meshheading:2178105-Cricetinae,
pubmed-meshheading:2178105-Cyclic AMP,
pubmed-meshheading:2178105-DNA,
pubmed-meshheading:2178105-Enzyme Activation,
pubmed-meshheading:2178105-Forskolin,
pubmed-meshheading:2178105-Gene Expression Regulation,
pubmed-meshheading:2178105-Humans,
pubmed-meshheading:2178105-Inositol Phosphates,
pubmed-meshheading:2178105-Phosphatidylinositol 4,5-Diphosphate,
pubmed-meshheading:2178105-Phosphatidylinositols,
pubmed-meshheading:2178105-Receptors, Thyrotropin,
pubmed-meshheading:2178105-Thyrotropin,
pubmed-meshheading:2178105-Transfection,
pubmed-meshheading:2178105-Type C Phospholipases
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pubmed:year |
1990
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pubmed:articleTitle |
Thyrotropin activates both the cyclic AMP and the PIP2 cascades in CHO cells expressing the human cDNA of TSH receptor.
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pubmed:affiliation |
Institute of Interdisciplinary Research, University of Brussels, School of Medicine, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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