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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1991-2-25
|
| pubmed:abstractText |
A continuous tumor cell line (LAP-35) was established from a primitive neuroectodermal tumor of bone from the right tibia of a 12-year-old female. The neural character of the cell line was documented by the spontaneous growth of neurites and by the presence of several neural markers, including neuron-specific enolase (NSE), S-100 protein, neurofilaments, chromogranin A, synaptophysin and positivity to monoclonal antibodies UJ127.11, UJ13A, UJ181.4. Cell-sorter analysis showed a high expression of nerve growth factor receptor (NGFr) and major histocompatibility complex class I-related molecules. A unique cytogenetic profile was observed, including a reciprocal chromosomal translocation (rct) 11:22 (q24;q12), typically associated with Ewing's sarcoma and neuroepithelioma, and deletion of the short arm of chromosome 1 (lp-), otherwise a feature of neuroblastoma. N-myc proto-oncogene was neither amplified nor expressed, whereas the expression of c-myc was documented by northern blot analysis. These features distinguish this new cell line from previously reported neuroectodermal cell lines, identifying LAP-35 as a unique model of a group of neural bone tumors that share characteristics of neuroblastoma as well as neuroepithelioma.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0737-1454
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
N
|
| pubmed:pagination |
409-24
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:2177080-Animals,
pubmed-meshheading:2177080-Bone Neoplasms,
pubmed-meshheading:2177080-Cell Division,
pubmed-meshheading:2177080-Child,
pubmed-meshheading:2177080-Female,
pubmed-meshheading:2177080-Flow Cytometry,
pubmed-meshheading:2177080-Fluorescent Antibody Technique,
pubmed-meshheading:2177080-Genes, myc,
pubmed-meshheading:2177080-Humans,
pubmed-meshheading:2177080-Immunohistochemistry,
pubmed-meshheading:2177080-Karyotyping,
pubmed-meshheading:2177080-Mice,
pubmed-meshheading:2177080-Mice, Nude,
pubmed-meshheading:2177080-Microscopy, Electron,
pubmed-meshheading:2177080-Neoplasm Transplantation,
pubmed-meshheading:2177080-Neoplasms, Nerve Tissue,
pubmed-meshheading:2177080-Tumor Cells, Cultured
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Establishment and characterization of a primitive neuroectodermal tumor of bone continuous cell line (LAP-35).
|
| pubmed:affiliation |
G. Prodi Interdepartmental Center for Cancer Research, Bologna, Italy.
|
| pubmed:publicationType |
Journal Article
|