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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12A
|
| pubmed:dateCreated |
1991-2-19
|
| pubmed:abstractText |
Intramolecular and intermolecular snRNA cross-links were generated by irradiating HeLa nuclear extract with 365 nm light in the presence of the psoralen derivative AMT. After deproteinization, cross-linked RNAs were resolved by gel electrophoresis and identified as anomalously migrating species by Northern blotting. In addition to the U4/U6 snRNA cross-link, we detected an intermolecular U2/U6 cross-link, as well as several apparently intramolecular U1, U2, and U5 cross-links. Photoreversal of the U2/U6 cross-link with 254 nm irradiation released stoichiometric amounts of U2 and U6 snRNA. To localize the U2/U6 cross-link, the 3' end of U2 in the purified U2/U6 complex was labeled selectively using a novel oligonucleotide "splint" technique. The labeled U2/U6 complex was then subjected to rapid enzymatic RNA sequencing or to targeted digestion of the U2 and U6 components of the complex by RNase H and a panel of complementary oligonucleotides. The U2/U6 cross-link is located upstream of nucleotide 15 in U2 and downstream of nucleotide 85 in U6, suggesting that the phylogenetically conserved base-pairing between these regions (6 consecutive base pairs in human, Drosophila melanogaster, and Caenorhabditis elegans, 7 in Schizosaccharomyces pombe and Trypanasoma brucei, 8 in Pisum sativum, 11 in Saccharomyces cerevisiae) is significant.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Small Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonuclease H,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, Small Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Trioxsalen,
http://linkedlifedata.com/resource/pubmed/chemical/aminomethyltrioxsalen
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0890-9369
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2146-56
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:2176635-Animals,
pubmed-meshheading:2176635-Base Composition,
pubmed-meshheading:2176635-Base Sequence,
pubmed-meshheading:2176635-Blotting, Northern,
pubmed-meshheading:2176635-Cross-Linking Reagents,
pubmed-meshheading:2176635-Endoribonucleases,
pubmed-meshheading:2176635-HeLa Cells,
pubmed-meshheading:2176635-Humans,
pubmed-meshheading:2176635-Molecular Sequence Data,
pubmed-meshheading:2176635-Nucleic Acid Conformation,
pubmed-meshheading:2176635-Oligonucleotides,
pubmed-meshheading:2176635-Phylogeny,
pubmed-meshheading:2176635-RNA, Small Nuclear,
pubmed-meshheading:2176635-RNA Splicing,
pubmed-meshheading:2176635-Ribonuclease H,
pubmed-meshheading:2176635-Ribonucleoproteins,
pubmed-meshheading:2176635-Ribonucleoproteins, Small Nuclear,
pubmed-meshheading:2176635-Trioxsalen
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Evidence for base-pairing between mammalian U2 and U6 small nuclear ribonucleoprotein particles.
|
| pubmed:affiliation |
Department of Molecular Biophysics and Biochemistry, Yale University School of Medicine, New Haven, Connecticut 06510-8024.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|