Linked Life Data

21766144

Source:http://linkedlifedata.com/resource/pubmed/id/21766144

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-7-18
pubmed:abstractText
Fourier transform infrared spectroscopy (FTIR) and cryomicroscopy were used to study the effects of dimethyl sulfoxide and ethylene glycol on cell pellets of human pulmonary microvascular endothelial cells during freezing from 4 degree C to -60 degree C at 1 degree C per min. FTIR analysis showed that membranes undergo a phase change in the presence of cryoprotective agents (CPAs) which was not observed in the absence of CPAs. Cryomicroscopy revealed the formation of intracellular ice and concomitant cell volume changes. Intracellular ice was detected in the majority of the cells both in the presence and absence of CPAs. Membrane phase changes were found to be most pronounced at intermediate concentrations of cryoprotective agents; for dimethyl sulfoxide at around 1 M and for ethylene glycol at around 1.5 M. At those concentrations cell survival after thawing exhibited a maximum. The results indicate that CPAs promote rather than prevent cell dehydration during freezing.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0143-2044
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
148-57
pubmed:meshHeading
pubmed:articleTitle
Dimethyl sulfoxide and ethylene glycol promote membrane phase change during cryopreservation.
pubmed:affiliation
Institute for Multiphase Processes, Leibniz Universitaet Hannover, Germany. spindler@imp.uni-hannover.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't