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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-7-18
pubmed:abstractText
In animals, oral administration of the cholera toxin B (CTB) subunit conjugated to the autoantigen insulin enhances the specific immune-unresponsive state. This is called oral tolerance and is capable of suppressing autoimmune type 1 diabetes (T1D). However, the process by which the CTB-insulin (CTB-INS) protein works as a therapy for T1D in vivo remains unclear. Here, we successfully expressed a green fluorescent protein- (GFP-) tagged CTB-Ins (CTB-Ins-GFP) fusion protein in silkworms in a pentameric form that retained the native ability to activate the mechanism. Oral administration of the CTB-Ins-GFP protein induced special tolerance, delayed the development of diabetic symptoms, and suppressed T1D onset in nonobese diabetic (NOD) mice. Moreover, it increased the numbers of CD4?CD25?Foxp3? T regulatory (Treg) cells in peripheral lymph tissues and affected the biological activity of spleen cells. This study demonstrated that the CTB-Ins-GFP protein produced in silkworms acted as an oral protein vaccine, inducing immunological tolerance involving CD4?CD25?Foxp3? Treg cells in treating T1D.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-10225827, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-10745159, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-11394960, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-11899420, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-12390307, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-14599847, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-16024127, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-16248282, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-16508971, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-16628077, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-16796692, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-16918694, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-17162357, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-17337769, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-17573530, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-17635805, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-17962948, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-18540865, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-18754812, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-18764920, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-19364533, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-19389426, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-1946445, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-19697097, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-19907495, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-20399761, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-20406664, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-2052603, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-20546263, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-20601187, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-21050716, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-3061520, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-7525393, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-7526379, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-7840857, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-7969282, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-8261794, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-8610965, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-8682203, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-8707060, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-8756716, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-8816970, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-8885998, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-9114038, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-9237797, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-9267984, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-9350452, http://linkedlifedata.com/resource/pubmed/commentcorrection/21765853-9423288
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1740-2530
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2011
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
831704
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:21765853-Administration, Oral, pubmed-meshheading:21765853-Adoptive Transfer, pubmed-meshheading:21765853-Animals, pubmed-meshheading:21765853-Autoantigens, pubmed-meshheading:21765853-Baculoviridae, pubmed-meshheading:21765853-Blotting, Western, pubmed-meshheading:21765853-Bombyx, pubmed-meshheading:21765853-Cholera Toxin, pubmed-meshheading:21765853-Diabetes Mellitus, Type 1, pubmed-meshheading:21765853-Disease-Free Survival, pubmed-meshheading:21765853-Female, pubmed-meshheading:21765853-Genetic Vectors, pubmed-meshheading:21765853-Green Fluorescent Proteins, pubmed-meshheading:21765853-Immune Tolerance, pubmed-meshheading:21765853-Immunization, pubmed-meshheading:21765853-Insulin, pubmed-meshheading:21765853-Mice, pubmed-meshheading:21765853-Mice, Inbred NOD, pubmed-meshheading:21765853-Mice, SCID, pubmed-meshheading:21765853-Recombinant Fusion Proteins, pubmed-meshheading:21765853-Spleen, pubmed-meshheading:21765853-T-Lymphocytes, Regulatory
pubmed:year
2011
pubmed:articleTitle
Protection against autoimmune diabetes by silkworm-produced GFP-tagged CTB-insulin fusion protein.
pubmed:affiliation
Institute of Biochemistry, College of Life Sciences, Zhejiang University (Zijingang Campus), 388 Yuhangtang Road, Hangzhou 310058, China.
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