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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2011-7-27
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pubmed:abstractText |
DNA methylation-dependent epigenetic mechanisms underlie the development and function of the mammalian brain. MeCP2 is highly expressed in neurons and functions as a molecular linker between DNA methylation, chromatin remodeling and transcription regulation. Previous in vitro studies have shown that neuronal activity-induced phosphorylation (NAIP) of methyl CpG-binding protein 2 (MeCP2) precedes its release from the Bdnf promoter and the ensuing Bdnf transcription. However, the in vivo function of this phosphorylation event remains elusive. We generated knock-in mice that lack NAIP of MeCP2 and found that they performed better in hippocampus-dependent memory tests, presented enhanced long-term potentiation at two synapses in the hippocampus and showed increased excitatory synaptogenesis. At the molecular level, the phospho-mutant MeCP2 protein bound more tightly to several MeCP2 target gene promoters and altered the expression of these genes. Our results suggest that NAIP of MeCP2 is required for modulating dynamic functions of the adult mouse brain.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Anesthetics, Local,
http://linkedlifedata.com/resource/pubmed/chemical/Dlgh4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Mecp2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Methyl-CpG-Binding Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neuronal Apoptosis-Inhibitory...,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphopyruvate Hydratase,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Serine,
http://linkedlifedata.com/resource/pubmed/chemical/Slc17a6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Glutamate Transport...
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1546-1726
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1001-8
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pubmed:meshHeading |
pubmed-meshheading:21765426-Analysis of Variance,
pubmed-meshheading:21765426-Anesthetics, Local,
pubmed-meshheading:21765426-Animals,
pubmed-meshheading:21765426-Biophysics,
pubmed-meshheading:21765426-Cells, Cultured,
pubmed-meshheading:21765426-Cerebral Cortex,
pubmed-meshheading:21765426-Electric Stimulation,
pubmed-meshheading:21765426-Excitatory Postsynaptic Potentials,
pubmed-meshheading:21765426-Fear,
pubmed-meshheading:21765426-Gene Expression Regulation,
pubmed-meshheading:21765426-Guanylate Kinase,
pubmed-meshheading:21765426-Hippocampus,
pubmed-meshheading:21765426-Long-Term Potentiation,
pubmed-meshheading:21765426-Maze Learning,
pubmed-meshheading:21765426-Membrane Proteins,
pubmed-meshheading:21765426-Memory,
pubmed-meshheading:21765426-Methyl-CpG-Binding Protein 2,
pubmed-meshheading:21765426-Mice,
pubmed-meshheading:21765426-Mice, Transgenic,
pubmed-meshheading:21765426-Microscopy, Confocal,
pubmed-meshheading:21765426-Nerve Tissue Proteins,
pubmed-meshheading:21765426-Neuronal Apoptosis-Inhibitory Protein,
pubmed-meshheading:21765426-Neurons,
pubmed-meshheading:21765426-Phosphopyruvate Hydratase,
pubmed-meshheading:21765426-Phosphorylation,
pubmed-meshheading:21765426-Potassium Chloride,
pubmed-meshheading:21765426-Serine,
pubmed-meshheading:21765426-Space Perception,
pubmed-meshheading:21765426-Swimming,
pubmed-meshheading:21765426-Synapses,
pubmed-meshheading:21765426-Tetrodotoxin,
pubmed-meshheading:21765426-Vesicular Glutamate Transport Protein 2
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pubmed:year |
2011
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pubmed:articleTitle |
Loss of activity-induced phosphorylation of MeCP2 enhances synaptogenesis, LTP and spatial memory.
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pubmed:affiliation |
Genetics Training Program, University of Wisconsin-Madison, Madison, Wisconsin, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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