Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6040
pubmed:dateCreated
2011-7-18
pubmed:databankReference
pubmed:abstractText
Fanconi anemia is a cancer predisposition syndrome caused by defects in the repair of DNA interstrand cross-links (ICLs). Central to this pathway is the Fanconi anemia I-Fanconi anemia D2 (FANCI-FANCD2) (ID) complex, which is activated by DNA damage-induced phosphorylation and monoubiquitination. The 3.4 angstrom crystal structure of the ~300 kilodalton ID complex reveals that monoubiquitination and regulatory phosphorylation sites map to the I-D interface, suggesting that they occur on monomeric proteins or an opened-up complex and that they may serve to stabilize I-D heterodimerization. The 7.8 angstrom electron-density map of FANCI-DNA crystals and in vitro data show that each protein has binding sites for both single- and double-stranded DNA, suggesting that the ID complex recognizes DNA structures that result from the encounter of replication forks with an ICL.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1095-9203
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
333
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
312-6
pubmed:meshHeading
pubmed-meshheading:21764741-Amino Acid Sequence, pubmed-meshheading:21764741-Animals, pubmed-meshheading:21764741-Binding Sites, pubmed-meshheading:21764741-Crystallography, X-Ray, pubmed-meshheading:21764741-DNA, pubmed-meshheading:21764741-DNA, Single-Stranded, pubmed-meshheading:21764741-DNA Repair, pubmed-meshheading:21764741-Fanconi Anemia, pubmed-meshheading:21764741-Fanconi Anemia Complementation Group D2 Protein, pubmed-meshheading:21764741-Fanconi Anemia Complementation Group Proteins, pubmed-meshheading:21764741-Hydrophobic and Hydrophilic Interactions, pubmed-meshheading:21764741-Mice, pubmed-meshheading:21764741-Models, Molecular, pubmed-meshheading:21764741-Molecular Sequence Data, pubmed-meshheading:21764741-Phosphorylation, pubmed-meshheading:21764741-Protein Binding, pubmed-meshheading:21764741-Protein Conformation, pubmed-meshheading:21764741-Protein Folding, pubmed-meshheading:21764741-Protein Structure, Secondary, pubmed-meshheading:21764741-Protein Structure, Tertiary, pubmed-meshheading:21764741-Static Electricity, pubmed-meshheading:21764741-Ubiquitin, pubmed-meshheading:21764741-Ubiquitination
pubmed:year
2011
pubmed:articleTitle
Structure of the FANCI-FANCD2 complex: insights into the Fanconi anemia DNA repair pathway.
pubmed:affiliation
Structural Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural