Source:http://linkedlifedata.com/resource/pubmed/id/21762807
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2011-7-18
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pubmed:abstractText |
Size polydispersity of immature human immunodeficiency virus type 1 (HIV-1) particles represents a challenge for traditional methods of biological ultrastructural analysis. An in vitro model for immature HIV-1 particles constructed from recombinant Gag proteins lacking residues 16-99 and the p6 domain assembled around spherical nanoparticles functionalized with DNA. This template-directed assembly approach led to a significant reduction in size polydispersity and revealed previously unknown structural features of immature-like HIV-1 particles. Electron microscopy and image reconstruction of these particles suggest that the Gag shell formed from different protein regions that are connected by a "scar"-an extended defect connecting the edges of two continuous, regularly packed protein layers. Thus, instead of a holey protein array, the experimental model presented here appears to consist of a continuous array of ?5000 proteins enveloping the core, in which regular regions are separated by extended areas of disorder.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1089-8638
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pubmed:author | |
pubmed:copyrightInfo |
Copyright © 2011 Elsevier Ltd. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:day |
22
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pubmed:volume |
410
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
667-80
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pubmed:meshHeading |
pubmed-meshheading:21762807-HIV-1,
pubmed-meshheading:21762807-Humans,
pubmed-meshheading:21762807-Hydrodynamics,
pubmed-meshheading:21762807-Image Processing, Computer-Assisted,
pubmed-meshheading:21762807-Metal Nanoparticles,
pubmed-meshheading:21762807-Recombinant Proteins,
pubmed-meshheading:21762807-Spectrophotometry, Ultraviolet,
pubmed-meshheading:21762807-Surface Properties,
pubmed-meshheading:21762807-Templates, Genetic,
pubmed-meshheading:21762807-Virion,
pubmed-meshheading:21762807-gag Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2011
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pubmed:articleTitle |
Structure and stoichiometry of template-directed recombinant HIV-1 Gag particles.
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pubmed:affiliation |
Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, N.I.H., Extramural,
Research Support, N.I.H., Intramural
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