Linked Life Data

21761326

Source:http://linkedlifedata.com/resource/pubmed/id/21761326

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2011-7-15
pubmed:abstractText
Laser-capture microdissection (LCM) is an excellent tool to selectively obtain target tissue or cells. The endolymphatic sac (ES) is part of the inner ear, and a large part of the ES is located in the temporal bone. The rat ES is conventionally harvested using stereomicroscopy. In this method, contamination is unavoidable because of its size and location; therefore, additional checks, such as in situ hybridization, are necessary to confirm the cellular localization, and quantitative analysis is difficult in the ES. We have shown a selective epithelial tissue method using LCM to obtain RNA without contamination from ES epithelial tissue.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1940-6029
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
755
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
441-8
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Specific RNA collection from the rat endolymphatic sac by laser-capture microdissection (LCM): LCM of a very small organ surrounded by bony tissues.
pubmed:affiliation
Department of Otolaryngology, Faculty of Medicine, Kagawa University, Takamatsu, Kagawa, Japan. kakaka@med.kagawa-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't