|
pubmed:abstractText |
We previously found that downregulation of protein kinase C? (PKC?) can inhibit retinal pigment epithelium (RPE) cell proliferation involved in the development of proliferative vitreoretinopathy (PVR). In this study, we tested whether PKC? could be downregulated via small interfering RNA (siRNA)-PKC? released from foldable capsular vitreous body (FCVB) in cultured human RPE cells. SiRNA-PKC? content, determined by ultraviolet (UV) spectrophotometer, was released from FCVB containing 200, 300, 400, 500, and 600 nm siRNA-PKC? in a time-dependent manner from 1 to 96 hours and a dose-dependent manner at five concentrations. The content (y) had a good linear relationship with time (x), especially in the 600 nm siRNA-PKC? group (y = 16.214x, R(2) = 0.9809). After treatment with siRNA-PKC? released from FCVBs, the PKC? was significantly decreased by RT-PCR, Western blot, and immunofluorescence analysis in RPE cells. These results indicate that PKC? was significantly downregulated by siRNA-PKC? released from FCVB in human RPE cells and provide us with a new avenue to prevent PVR.
|
