Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2011-7-11
pubmed:abstractText
The hair follicle consists of several distinctive epidermal cell layers. The hair root, which undergoes keratinization, is surrounded by two sheaths: the inner root sheath (IRS) and the outer root sheath (ORS). The ORS is continuous with the basal layer of the epidermis. Its cells do not keratinize in situ, unlike IRS. We have previously demonstrated that keratinization of the ORS was prevented by contact with the IRS in hair follicle mid-segments (i.e. fragments dissected from skin at the level above the hair bulb and below the opening of the sebaceous gland duct) cultured on agarose layer. The purpose of this study was to determine whether the same applies to the hair bulb. After isolation, intact bulbs or hair bulb-derived cells were incubated in suspension in a low or high calcium medium. The level of mRNA for differentiation markers: involucrin, filaggrin, keratinocyte differentiation associated protein and trichohyalin, was studied by RealTime PCR. We observed increased Ca(2+) upregulated expression of involucrin, filaggrin, trichohyalin and Kdap in cultures of bulb-derived cells, but in hair bulbs downregulation of involucrin and trichohyalin was observed. We concluded that the inner root sheath exerts an inhibitory effect on the expression of involucrin and trichohyalin already in the hair bulbs. The observation that downregulation of involucrin expression under Ca(2+) influence occurs both in hair bulb and midsegments could simplify future experiments, since their separation does not seem to be necessary.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid Endopeptidases, http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, http://linkedlifedata.com/resource/pubmed/chemical/Intermediate Filament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Kdap protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Sepharose, http://linkedlifedata.com/resource/pubmed/chemical/filaggrin, http://linkedlifedata.com/resource/pubmed/chemical/involucrin, http://linkedlifedata.com/resource/pubmed/chemical/trichohyalin
pubmed:status
MEDLINE
pubmed:issn
1897-5631
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
335-43
pubmed:meshHeading
pubmed-meshheading:21744336-Animals, pubmed-meshheading:21744336-Aspartic Acid Endopeptidases, pubmed-meshheading:21744336-Biological Markers, pubmed-meshheading:21744336-Calcium, pubmed-meshheading:21744336-Cell Differentiation, pubmed-meshheading:21744336-Cell Shape, pubmed-meshheading:21744336-Cells, Cultured, pubmed-meshheading:21744336-Culture Media, pubmed-meshheading:21744336-Down-Regulation, pubmed-meshheading:21744336-Female, pubmed-meshheading:21744336-Hair Follicle, pubmed-meshheading:21744336-Immunohistochemistry, pubmed-meshheading:21744336-Intermediate Filament Proteins, pubmed-meshheading:21744336-Keratinocytes, pubmed-meshheading:21744336-Protein Precursors, pubmed-meshheading:21744336-RNA, Messenger, pubmed-meshheading:21744336-Rats, pubmed-meshheading:21744336-Rats, Wistar, pubmed-meshheading:21744336-Sepharose
pubmed:year
2011
pubmed:articleTitle
Involucrin, but not filaggrin and Kdap mRNA, expression is downregulated in 3-D cultures of intact rat hair bulbs after calcium stimulation.
pubmed:affiliation
Department of Histology and Embryology, Medical University of Warsaw, Poland. justynan@ib.amwaw.edu.pl
pubmed:publicationType
Journal Article