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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2011-9-12
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pubmed:abstractText |
Cultured retinal pigment epithelium (RPE) may become a therapeutic option for transplantation in retinal disease. However maintaining a native RPE phenotype in vitro has proven challenging. The human RPE cell-line ARPE-19 is used widely as an alternative to primary RPE. It is grown in DMEM/F12 medium as standard, but its phenotype is dependent on culture conditions, and many differentiation markers are usually absent. The purpose of this study was to examine how this sensitive phenotype of ARPE-19 can be modulated by growth media with or without the metabolite pyruvate to elucidate better RPE growth conditions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/11-cis-retinal-binding protein,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media,
http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MERTK protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pyruvic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/RPE65 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/VEGFA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A
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pubmed:status |
MEDLINE
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pubmed:issn |
1552-5783
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pubmed:author |
pubmed-author:AhmadoAhmadA,
pubmed-author:CarrAmanda-JayneAJ,
pubmed-author:ChenFred KFK,
pubmed-author:ChenLi LiLL,
pubmed-author:CoffeyPeter JPJ,
pubmed-author:GiasCarlosC,
pubmed-author:LawrenceJean MJM,
pubmed-author:SemoMa'ayanM,
pubmed-author:TurowskiPatricP,
pubmed-author:VuglerAnthony AAA,
pubmed-author:da CruzLyndonL
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pubmed:issnType |
Electronic
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pubmed:volume |
52
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7148-59
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pubmed:meshHeading |
pubmed-meshheading:21743014-Biological Markers,
pubmed-meshheading:21743014-Blotting, Western,
pubmed-meshheading:21743014-Carrier Proteins,
pubmed-meshheading:21743014-Cell Differentiation,
pubmed-meshheading:21743014-Cells, Cultured,
pubmed-meshheading:21743014-Culture Media,
pubmed-meshheading:21743014-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:21743014-Eye Proteins,
pubmed-meshheading:21743014-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:21743014-Humans,
pubmed-meshheading:21743014-Phagocytosis,
pubmed-meshheading:21743014-Phenotype,
pubmed-meshheading:21743014-Proto-Oncogene Proteins,
pubmed-meshheading:21743014-Pyruvic Acid,
pubmed-meshheading:21743014-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:21743014-Retinal Pigment Epithelium,
pubmed-meshheading:21743014-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:21743014-Vascular Endothelial Growth Factor A
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pubmed:year |
2011
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pubmed:articleTitle |
Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19.
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pubmed:affiliation |
Department of Ocular Biology and Therapeutics, University College London (UCL), Institute of Ophthalmology, London, United Kingdom. a.ahmado@ucl.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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