Source:http://linkedlifedata.com/resource/pubmed/id/21740442
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2011-9-2
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| pubmed:abstractText |
BALB/c is an inbred stress-sensitive mouse strain exhibiting low brain serotonin (5-HT) content and a 5-HT biosynthetic enzyme tryptophan hydroxylase (Tph2) variant reported to have lower catalytic activity compared with other inbred base strains. To evaluate other mechanisms that may explain low 5-HT, we compared BALB/cJ mice and a control inbred strain C57Bl/6J mice, for expression of Tph2 mRNA, TPH2 protein and regional levels of 5-HT and its metabolite 5-hydroxyindoleacetic acid. Tph2 mRNA and TPH2 protein in brainstem dorsal raphe nuclei was assayed by in situ hybridization and immunocytochemistry respectively. 5-HT and 5-hydroxyindoleacetic acid were determined by HPLC. BALB/cJ mice had 20% less Tph2 mRNA and 28% fewer TPH2 immunolabeled neurons than C57Bl/6J mice (t = -2.59, p = 0.02). The largest difference in Tph2 transcript expression was in rostral dorsal raphe nuclei (t = 2.731, p = 0.008). 5-HT was 15% lower in the midbrain and 18% lower in the cerebral cortex of BALB/cJ compared with C57Bl/6J mice (p < 0.05). The behavioral differences in BALB/cJ mice relative to the C57Bl/6J strain may be due in part, to fewer 5-HT neurons and lower Tph2 gene expression resulting in less 5-HT neurotransmission. Future studies quantifying expression per neuron are needed to determine whether less expression is explained by fewer neurons or also less expression per neuron, or both.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Sep
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| pubmed:issn |
1471-4159
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| pubmed:author | |
| pubmed:copyrightInfo |
© 2011 The Authors. Journal of Neurochemistry © 2011 International Society for Neurochemistry.
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| pubmed:issnType |
Electronic
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| pubmed:volume |
118
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1067-74
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| pubmed:meshHeading |
pubmed-meshheading:21740442-Animals,
pubmed-meshheading:21740442-Autoradiography,
pubmed-meshheading:21740442-Brain Chemistry,
pubmed-meshheading:21740442-Cell Count,
pubmed-meshheading:21740442-Densitometry,
pubmed-meshheading:21740442-Hydroxyindoleacetic Acid,
pubmed-meshheading:21740442-Image Processing, Computer-Assisted,
pubmed-meshheading:21740442-Immunohistochemistry,
pubmed-meshheading:21740442-In Situ Hybridization,
pubmed-meshheading:21740442-Male,
pubmed-meshheading:21740442-Mice,
pubmed-meshheading:21740442-Mice, Inbred BALB C,
pubmed-meshheading:21740442-Mice, Inbred C57BL,
pubmed-meshheading:21740442-Neurons,
pubmed-meshheading:21740442-Raphe Nuclei,
pubmed-meshheading:21740442-Serotonin,
pubmed-meshheading:21740442-Species Specificity,
pubmed-meshheading:21740442-Tryptophan Hydroxylase
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| pubmed:year |
2011
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| pubmed:articleTitle |
Neuronal tryptophan hydroxylase expression in BALB/cJ and C57Bl/6J mice.
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| pubmed:affiliation |
Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, New York 10032, USA. hb2104@columbia.edu
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| pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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