Linked Life Data

2173786

Source:http://linkedlifedata.com/resource/pubmed/id/2173786

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1990-12-31
pubmed:abstractText
Infection with human cytomegalovirus in the presence of the antiviral nucleotide analog ganciclovir results in continuing low-level viral DNA synthesis and the accumulation of relatively small fragments of double-stranded progency DNA. These fragments consistently proved to represent amplification of sequences from only one small section of the viral genome (EcoRI-V) lying near the center of the unique L segment. Further mapping revealed that the viral sequences represented in these fragments occurred in gradients of abundance that decreased in both directions from a point near 0.35 to 0.4 map unit. The proportion of amplified sequences increased with both time after infection and dosage of ganciclovir used. We conclude that the primary lytic cycle replication origin of human cytomegalovirus lies within a 3- to 4-kb region immediately upstream and to the right of the promoter for the single-stranded DNA-binding protein (DB140). The amplified origin-containing DNA molecules appeared to arise by continuing rounds of bidirectional initiation on truncated fragments of the genome that were generated as a result of chain termination effects induced by the incorporation of ganciclovir into the viral DNA. Inspection of the DNA sequence in the vicinity of ori-Lyt revealed a large complex upstream region that may be a noncoding intergenic domain and that bears no homology to any previously described herpesvirus origin. This 2.5-kb region includes many duplicated and inverted sequences, together with consensus CRE/ATF and other transcription factor-binding sites, and an interesting set of 23 copies of an interspersed decamer consensus element AAAACACCGT that is also conserved at the equivalent locus in simian cytomegalovirus. This work represents the first identification of an origin domain in a cytomegalovirus genome and is the first demonstration of a bidirectional mechanism for any herpesvirus lytic cycle origin.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-166202, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-178916, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-185428, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-194406, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-199705, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-209219, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2154599, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2161319, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-219240, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-228055, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2537958, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2538835, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2542579, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2542609, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2544294, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2545037, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2547525, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2549858, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2553730, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2826806, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2826807, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2831398, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2834723, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2840659, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2845124, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2846181, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2983224, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2986118, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2987682, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-2996781, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-3009865, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-3018528, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-3024166, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-3041036, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-3178814, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6090700, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6096489, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6268822, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6282691, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6287032, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6300290, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6313969, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6316638, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6325150, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6328526, http://linkedlifedata.com/resource/pubmed/commentcorrection/2173786-6329712
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:geneSymbol
Lyt, ori
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6184-95
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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