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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1990-12-24
|
| pubmed:abstractText |
The fatty acid composition of total lipids and the adrenoceptor-mediated activation of glycogenolysis were studied in isolated hepatocytes from rats maintained on a control diet or on an essential fatty acid (EFA)-free diet. In cells from rats on the EFA-free diet there was a marked reduction in linoleic and arachidonic acid (AA) contents and an increase in eicosatrienoic, oleic, and palmitoleic acid contents compared to controls. In freshly isolated cells from both groups, phosphorylase a activity was increased by phenylephrine or epinephrine but not by isoproterenol, and the effect of epinephrine was inhibited by phenoxybenzamine but not by propranolol. When control cells were preincubated in a serum-free buffer for 4 h before testing, the effect of phenylephrine on phosphorylase a activity was reduced, isoproterenol became a potent agonist and the effect of epinephrine was partially inhibited either by phenoxybenzamine or by propranolol. The emerging beta-adrenergic response in 4-h cells was associated with a marked potentiation of isoproterenol-induced cAMP accumulation. A similar 4-h preincubation of EFA-deficient cells resulted in a reduced response to phenylephrine while isoproterenol remained ineffective for increasing either phosphorylase a activity or cAMP production. The response of these 4-h cells to isoproterenol could be restored by in vivo replacement of the EFA-deficient diet with control diet for the last 4 weeks prior to the experiment, but not by the in vitro exposure of the EFA-deficient cells to 10 microM AA throughout the 4-h incubation period. Extending previous observations (Refs. (6-8)), the present results suggest that the time-dependent emergence of beta-adrenergic glycogenolysis, but not the parallel reduction of the alpha-adrenergic response, is mediated by AA or its metabolite(s), which probably act by facilitating the G-protein-dependent coupling of beta-receptors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Epinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Essential,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Unsaturated,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Liver Glycogen,
http://linkedlifedata.com/resource/pubmed/chemical/Melitten,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorylase a,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0003-9861
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
283
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
34-9
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| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:2173490-Animals,
pubmed-meshheading:2173490-Cells, Cultured,
pubmed-meshheading:2173490-Cyclic AMP,
pubmed-meshheading:2173490-Epinephrine,
pubmed-meshheading:2173490-Fatty Acids, Essential,
pubmed-meshheading:2173490-Fatty Acids, Unsaturated,
pubmed-meshheading:2173490-Female,
pubmed-meshheading:2173490-Forskolin,
pubmed-meshheading:2173490-Glucagon,
pubmed-meshheading:2173490-Isoproterenol,
pubmed-meshheading:2173490-Kinetics,
pubmed-meshheading:2173490-Liver,
pubmed-meshheading:2173490-Liver Glycogen,
pubmed-meshheading:2173490-Melitten,
pubmed-meshheading:2173490-Phenylephrine,
pubmed-meshheading:2173490-Phosphorylase a,
pubmed-meshheading:2173490-Pregnancy,
pubmed-meshheading:2173490-Rats,
pubmed-meshheading:2173490-Rats, Inbred Strains,
pubmed-meshheading:2173490-Receptors, Adrenergic,
pubmed-meshheading:2173490-Reference Values
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
The effect of essential fatty acid deficiency on the adrenergic activation of glycogenolysis in rat hepatocytes.
|
| pubmed:affiliation |
Laboratory of Physiologic and Pharmacologic Studies, National Institute on Alcohol Abuse and Alcoholism, Bethesda, Maryland 20892.
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| pubmed:publicationType |
Journal Article
|