2172968

umls-concept:C0006556, umls-concept:C0009015, umls-concept:C0033684, umls-concept:C0035668, umls-concept:C0086418, umls-concept:C0220905, umls-concept:C0679058, umls-concept:C1547699, umls-concept:C2700640

1990-12-4

Iron-responsive elements (IREs) are stemloop structures found in the mRNAs encoding ferritin and the transferrin receptor. These elements participate in the iron-induced regulation of the translation of ferritin and the stability of the transferrin receptor mRNA. Regulation in both instances is mediated by binding of a cytosolic protein to the IREs. High-affinity binding is seen when cells are starved of iron and results in repression of ferritin translation and inhibition of transferrin receptor mRNA degradation. The IRE-binding protein (IRE-BP) has been identified as an approximately 90-kDa protein that has been purified by both affinity and conventional chromatography. In this report we use RNA affinity chromatography and two-dimensional gel electrophoresis to isolate the IRE-BP for protein sequencing. A degenerate oligonucleotide probe derived from a single peptide sequence was used to isolate a cDNA clone that encodes a protein containing 13 other sequenced peptides obtained from the IRE-BP. Consistent with previous characterization of the IRE-BP, the cDNA encodes a protein of 87 kDa with a slightly acidic pI, and the corresponding mRNA of approximately 3.6 kilobases is found in a variety of cell types. The encoded protein contains a nucleotide-binding consensus sequence and regions of cysteine and histidine clusters. This mRNA is encoded by a single gene on human chromosome 9, a finding consistent with previous localization by functional mapping. The protein contains no previously defined consensus motifs for either RNA or DNA binding. The simultaneous cloning of a different, but highly homologous, cDNA suggests that the IRE-BP is a member of a distinct gene family.

http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2106665, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2153297, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2156853, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2157191, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2294594, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2296288, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2333293, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-236308, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2452485, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2467746, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2474819, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2498873, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2522817, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2553732, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2569464, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2583116, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2601708, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2683086, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2711187, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2752428, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2760024, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2765515, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2771641, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-2997226, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3124104, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3127826, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3162770, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3266604, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3291115, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3313383, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3370673, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3413484, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3479802, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-3685996, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-6092369, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/2172968-6802926

http://linkedlifedata.com/resource/pubmed/journal/7505876

http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/Ferritins, http://linkedlifedata.com/resource/pubmed/chemical/Iron, http://linkedlifedata.com/resource/pubmed/chemical/Iron-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Iron-Regulatory Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transferrin, http://linkedlifedata.com/resource/pubmed/chemical/ferritin receptor

Oct

pubmed-author:BurgessW HWH, pubmed-author:HaileD JDJ, pubmed-author:HarfordJ BJB, pubmed-author:KaptainSS, pubmed-author:KlausnerR DRD, pubmed-author:McBrideO WOW, pubmed-author:RouaultT ATA, pubmed-author:SamaniegoFF, pubmed-author:TangC KCK

87

Y

2009-11-18