21728824

Source:http://linkedlifedata.com/resource/pubmed/id/21728824

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0003320, umls-concept:C0011185, umls-concept:C0014441, umls-concept:C0229671, umls-concept:C0242485
pubmed:issue	4
pubmed:dateCreated	2011-7-6
pubmed:abstractText	Homologous and heterologous combinations of enzyme conjugate and antibody in steroid enzyme immunoassay (EIA) influences unlabeled steroid recognition by antibody that affects sensitivity of the assay. To develop dehydroepiandrosterone (DHEA) antigen heterologous enzyme linked immunosorbent assay (ELISA), antibodies were generated against DHEA-3-hemisuccinate-bovine serum albumin (DHEA-3-HS-BSA), DHEA-7-carboxymethyloxime-bovine serum albumin (DHEA-7-CMO-BSA), and DHEA-17-carboxymethyloxime-bovine serum albumin (DHEA-17-CMO-BSA). Five horseradish peroxidase (HRP) enzyme conjugates were prepared using five testosterone derivatives [testosterone-3-CMO (T-3-CMO), testosterone-17-HS (T-17-HS), testosterone-17-glucuronoside (T-17-G), testosterone-19-carboxymethylether (T-19-CME), and testosterone-11-HS (T-11-HS)]. Fifteen antigen heterologous combinations of antibody and enzyme conjugates were evaluated in the standard binding assay; only two combinations showed binding. The use of antigen heterologous combination (different antigen in label than the immunogen) resulted in development of a simple, direct, and convenient assay as it permits the direct addition of the serum sample into the assay and it requires only 1.5 h to complete.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/100963688
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/19-O-carboxymethoxytestosterone, http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Heterophile, http://linkedlifedata.com/resource/pubmed/chemical/Dehydroepiandrosterone, http://linkedlifedata.com/resource/pubmed/chemical/Horseradish Peroxidase, http://linkedlifedata.com/resource/pubmed/chemical/Oximes, http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine, http://linkedlifedata.com/resource/pubmed/chemical/Testosterone, http://linkedlifedata.com/resource/pubmed/chemical/dehydroepiandrosterone-7-O-(carboxym..., http://linkedlifedata.com/resource/pubmed/chemical/dehydroepiandrosterone-bovine..., http://linkedlifedata.com/resource/pubmed/chemical/testosterone-3-carboxymethyloxime
pubmed:status	MEDLINE
pubmed:month	Oct
pubmed:issn	1532-4230
pubmed:author	pubmed-author:ChaubeShail KSK, pubmed-author:KariyaKiran PKP, pubmed-author:KumarDineshD, pubmed-author:ShrivastavTulsidas GTG, pubmed-author:SinghRitaR
pubmed:issnType	Electronic
pubmed:volume	32
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	326-41
pubmed:meshHeading	pubmed-meshheading:21728824-Adjuvants, Immunologic, pubmed-meshheading:21728824-Animals, pubmed-meshheading:21728824-Antigens, Heterophile, pubmed-meshheading:21728824-Cattle, pubmed-meshheading:21728824-Dehydroepiandrosterone, pubmed-meshheading:21728824-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:21728824-Horseradish Peroxidase, pubmed-meshheading:21728824-Oximes, pubmed-meshheading:21728824-Sensitivity and Specificity, pubmed-meshheading:21728824-Serum Albumin, Bovine, pubmed-meshheading:21728824-Testosterone
pubmed:year	2011
pubmed:articleTitle	Antigen heterologous enzyme linked immunosorbent assay for the measurement of DHEA in serum.
pubmed:affiliation	Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, Munirka, New Delhi, India. tgsrivastava@nihfw.org
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies