2172800

pubmed:Citation

9

Denervation of rat ventral prostate has been accomplished by excising prostatic tissue fragments and implanting them under the renal capsules of intact syngeneic rats. This resulted in a substantial reduction of expression of a major organ-specific secretory protein, prostatic binding protein (PBP). The depressed level of PBP and its subunits and mRNAs could be restored, however, to as much as 80% of control levels by the administration of a pharmacological dose of exogenous androgen, testosterone propionate (TP), and/or a beta-adrenergic agonist, isoproterenol (ISO). Furthermore, compared to ascorbate-treated controls, TP and ISO increased the synthesis of total cellular protein and PBP by the prostatic renal implants. TP and/or ISO also remodelled the luminal epithelial structure and elevated secretory functions. ISO alone had no effect, however, in castrated animals, indicating that androgen plays a dominant role in the restoration of tissue PBP content. Concomitant to increased PBP content and remodelling of prostatic histomorphology, androgen was also found to raise the depressed levels of beta 2-adrenergic and androgen receptors in the prostatic isografts maintained in intact hosts. In contrast, although an established rat prostatic epithelial cell line (NbE-1) contains high affinity androgen receptor, androgen failed to restore beta-adrenergic receptor as well as PBP content in this cultured cell line. These results, taken together, suggest that a tight coupling between androgen receptor and beta 2-adrenergic receptor pathways may be a prerequisite for PBP expression and functional differentiation in the rat ventral prostate gland.

eng

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MEDLINE

0888-8809

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1343-53

pubmed-meshheading:2172800-Androgen-Binding Protein, pubmed-meshheading:2172800-Androgens, pubmed-meshheading:2172800-Animals, pubmed-meshheading:2172800-Blotting, Western, pubmed-meshheading:2172800-Gene Expression Regulation, pubmed-meshheading:2172800-Isoproterenol, pubmed-meshheading:2172800-Kidney, pubmed-meshheading:2172800-Male, pubmed-meshheading:2172800-Prostate, pubmed-meshheading:2172800-Prostatein, pubmed-meshheading:2172800-RNA, Messenger, pubmed-meshheading:2172800-Rats, pubmed-meshheading:2172800-Rats, Inbred Strains, pubmed-meshheading:2172800-Rats, Inbred WF, pubmed-meshheading:2172800-Receptors, Adrenergic, beta, pubmed-meshheading:2172800-Receptors, Androgen, pubmed-meshheading:2172800-Secretoglobins, pubmed-meshheading:2172800-Testosterone, pubmed-meshheading:2172800-Uteroglobin

Regulation of gene expression in rat prostate by androgen and beta-adrenergic receptor pathways.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't