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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2011-7-19
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pubmed:abstractText |
Whole-mount in situ hybridization (WISH) is extensively used to characterize gene expression patterns in developing and adult brain and other tissues. To obtain an idea whether a novel gene might be involved in specification of a distinct brain subdivision, nucleus or neuronal lineage, it is often useful to correlate its expression with that of a known regional or neuronal marker gene. Two-color fluorescent in situ hybridization (FISH) can be used to compare different transcript distributions at cellular resolution. Conventional two-color FISH protocols require two separate rounds of horseradish peroxidase (POD)-based transcript detection, which involves tyramide signal amplification (TSA) and inactivation of the first applied antibody-enzyme conjugate before the second detection round.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-10679465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-10704836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-10842361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-10948532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-11252185,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-11316436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-11316437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-11493543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-11911652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-11922991,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-11923201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-12948657,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-1352238,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-14991720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-15297669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-15456722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-16235555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-16369549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-16453446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-17210054,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-17912740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-18248170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-18641955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-20533356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-21037591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-2118160,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-21466670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-2476281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-2558138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-7507621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-7822768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-7940754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-8178366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-8269519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-8601698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-8909126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-8909127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-9533954,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21726453-9603790
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1471-213X
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43
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pubmed:meshHeading |
pubmed-meshheading:21726453-Alkaline Phosphatase,
pubmed-meshheading:21726453-Animals,
pubmed-meshheading:21726453-Brain,
pubmed-meshheading:21726453-Embryo, Nonmammalian,
pubmed-meshheading:21726453-Fluoresceins,
pubmed-meshheading:21726453-Gene Expression Regulation, Developmental,
pubmed-meshheading:21726453-Horseradish Peroxidase,
pubmed-meshheading:21726453-In Situ Hybridization, Fluorescence,
pubmed-meshheading:21726453-Zebrafish
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pubmed:year |
2011
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pubmed:articleTitle |
Two-color fluorescent in situ hybridization in the embryonic zebrafish brain using differential detection systems.
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pubmed:affiliation |
Department of Biosciences and Nutrition, Karolinska Institutet, SE-141 83 Huddinge, Sweden.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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