Linked Life Data

21725819

Source:http://linkedlifedata.com/resource/pubmed/id/21725819

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2011-7-4
pubmed:abstractText
The effects of trimebutine maleate (TM) on spontaneous contractions of colonic longitudinal muscle were investigated in guinea pigs. The contractile responses of smooth muscle strips were recorded by an isometric force transducer. Membrane and action potentials were detected by an intracellular microelectrode technique. The whole-cell patch clamp recording technique was used to record the changes in large conductance Ca(2+)-activated K(+) (BK(ca)) and L-type Ca(2+) currents in colonic smooth muscle cells. At high concentrations (30, 100, and 300 ?M), TM inhibited the amplitude of spontaneous contractions. At low concentrations (1 and 10 ?M), TM attenuated the frequency and tone of smooth muscle strips, whereas TM had no influence on the amplitude of spontaneous contractions. TM depolarized the membrane potentials, but decreased the amplitude and frequency of action potentials at high concentrations. TM inhibited BK(ca) and L-type Ca(2+) currents in a dose-dependent manner. In the presence of the BK(ca) channel opener, NS1619, TM also inhibited BK(ca) currents. Bayk8644, a L-type Ca(2+) channel opener, increased L-type Ca(2+) currents. This augmentation was also attenuated by TM. These results suggest that TM attenuates intestinal motility through inhibition of L-type Ca(2+) currents, and depolarizes membrane potentials by reducing BK(ca) currents. Thus, TM may be a multiple-ion channel regulator in the gastrointestinal tract.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0253-6269
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
979-85
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
Effects of trimebutine maleate on colonic motility through Ca²+-activated K+ channels and L-type Ca²+ channels.
pubmed:affiliation
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan 430060, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't