Linked Life Data

2172086

Source:http://linkedlifedata.com/resource/pubmed/id/2172086

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1990-12-4
pubmed:abstractText
The construction of plasmids which over-produce the Escherichia coli DNA gyrase A and B proteins (GyrA and GyrB) is described. Both plasmids are based on the pTTQ vectors of Stark [Gene 51 (1987) 255-267] and contain either the gyrA or gyrB gene under the tight control of the hybrid tac promoter. Expression of the gyrase genes is shown to be repressed in the absence of the inducer IPTG, but in its presence, strains containing these plasmids synthesise the A and B proteins to about 40% of soluble cell protein.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
93
pubmed:geneSymbol
gyrB
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
139-42
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Cloning of the DNA gyrase genes under tac promoter control: overproduction of the gyrase A and B proteins.
pubmed:affiliation
Department of Biochemistry, University of Leicester, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't