Linked Life Data

21717341

Source:http://linkedlifedata.com/resource/pubmed/id/21717341

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2011-6-30
pubmed:abstractText
In Escherichia coli, primary rRNA transcripts must be processed by a complex process in which several ribonucleases are involved in order to generate mature 16S, 23S, and 5S rRNA molecules. While it is known that RNase G, a single-stranded RNA-specific endoribonuclease encoded by the rng gene, plays an active role in the maturation of the 5'-end of 16S rRNA, its involvement in the maturation of the 5'-end of 23S rRNA remains unclear. Here we show that E. coli cells deleted for the rng gene accumulate the 23S rRNA precursor containing an extra 77 nucleotides at its mature 5'-end. In vitro cleavage assays show that RNase G cleaves synthetic RNA containing a sequence encompassing the 5'-end to 77 nucleotides upstream of mature 23S rRNA at two sites present in single-stranded regions. Our results suggest the involvement of RNase G in the processing of the 5'-region of 23S rRNA precursors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1976-3794
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
49
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
508-11
pubmed:meshHeading
pubmed:year
2011
pubmed:articleTitle
RNase G participates in processing of the 5'-end of 23S ribosomal RNA.
pubmed:affiliation
School of Biological Sciences, Chung-Ang University, Seoul, 156-756, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't