Linked Life Data

2171646

Source:http://linkedlifedata.com/resource/pubmed/id/2171646

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1990-12-4
pubmed:abstractText
Three cell types were isolated from dissociated 16-cell sea urchin embryos. Four membrane density fractions from discontinuous gradients have different proportions of lipids, surfacer markers and enzymes for the three cell types. Assays of lipid content, CH/PLIPID and SPH/PC ratios, acyl chain length, level of unsaturation by proton NMR and assays of enzyme activity revealed variation at the same density between the three cell types and among different densities from one cell type. There were also differences between whole embryos and dissociated embryo cells. There was no typical membrane domain at a particular density common to the cell types. Cell surface characteristics and polarity of adult cells rely on which lipid domains and enzymes are present, their association with cytoskeleton and how they are localized. At the 16-cell stage these characteristics are still very dynamic as revealed by cytochemical localization of Na+/K(+)-ATPase which varied with cell type and suggests endocytosis at set times in the division cycle. Polarity has not been permanently set for Na+/K(+)-ATPase yet. Membrane enzyme and lipid distributions unique to the three cell types seen in this study suggest parcelling out or insertion of new membrane domains occurs during early sea urchin cleavage. Perturbation of membrane density distribution and lipid content occurs after treatment of embryos with animalizing and vegetalizing teratogens which alter development.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
1028
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
117-40
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Membrane fractions display different lipid and enzyme content in three cell types in 16-cell stage embryos of sea urchins.
pubmed:affiliation
Biology Department, California State University, Northridge 91330.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't