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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
28
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pubmed:dateCreated |
2011-7-13
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pubmed:abstractText |
A triazole mimic of a DNA phosphodiester linkage has been produced by templated chemical ligation of oligonucleotides functionalized with 5'-azide and 3'-alkyne. The individual azide and alkyne oligonucleotides were synthesized by standard phosphoramidite methods and assembled using a straightforward ligation procedure. This highly efficient chemical equivalent of enzymatic DNA ligation has been used to assemble a 300-mer from three 100-mer oligonucleotides, demonstrating the total chemical synthesis of very long oligonucleotides. The base sequences of the DNA strands containing this artificial linkage were copied during PCR with high fidelity and a gene containing the triazole linker was functional in Escherichia coli.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-11381035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-11975567,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-12203546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-12375897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-12530893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-15049637,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-15934050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-16182964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-16312051,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-16448080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-16464004,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-16898780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-17488075,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-18418819,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-18656947,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-19292490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-19760115,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-20222824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-20309492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-20488990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-20713730,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/21709264-9857206
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
12
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pubmed:volume |
108
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11338-43
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pubmed:meshHeading |
pubmed-meshheading:21709264-Base Sequence,
pubmed-meshheading:21709264-DNA,
pubmed-meshheading:21709264-DNA-Directed DNA Polymerase,
pubmed-meshheading:21709264-Escherichia coli,
pubmed-meshheading:21709264-Molecular Mimicry,
pubmed-meshheading:21709264-Molecular Sequence Data,
pubmed-meshheading:21709264-Molecular Structure,
pubmed-meshheading:21709264-Oligodeoxyribonucleotides,
pubmed-meshheading:21709264-Polymerase Chain Reaction,
pubmed-meshheading:21709264-Sequence Homology, Nucleic Acid,
pubmed-meshheading:21709264-Triazoles
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pubmed:year |
2011
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pubmed:articleTitle |
Biocompatible artificial DNA linker that is read through by DNA polymerases and is functional in Escherichia coli.
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pubmed:affiliation |
School of Chemistry, University of Southampton, Highfield, Southampton SO17 1BJ, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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