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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1990-11-21
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pubmed:abstractText |
The regulation of human IFN-gamma receptor (IFN-gamma-R) expression by granulocyte-macrophage CSF (GM-CSF) was investigated. On monocytic cell lines (U937, HL60) and peripheral blood monocytes, IFN-gamma-binding capacity was down-regulated upon incubation with GM-CSF. Scatchard plot analyses revealed that down-regulation was caused by a decrease in IFN-gamma-R number rather than by a change in affinity. GM-CSF treatment did not reduce IFN-gamma-R-specific mRNA levels, but reduced the half-life of membrane-expressed IFN-gamma-R, indicating a post-translational control of IFN-gamma-R by GM-CSF. Because both IFN-gamma and GM-CSF are crucially involved in activation of monocytic function, the data presented suggest that down-regulation of IFN-gamma-R by GM-CSF may represent a potential negative feedback control of monocyte activation. Further studies of IFN-gamma binding characteristics and isolation of IFN-gamma-R by immunoprecipitation revealed that IFN-gamma binding to human peripheral blood monocytes is mediated by a receptor protein structurally and functionally identical to that previously characterized in several established cell lines of other tissue origin.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interferon,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Tumor Necrosis Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
145
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2914-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:2170527-Blotting, Northern,
pubmed-meshheading:2170527-Cell Line,
pubmed-meshheading:2170527-Cell Membrane,
pubmed-meshheading:2170527-Down-Regulation,
pubmed-meshheading:2170527-Gene Expression,
pubmed-meshheading:2170527-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:2170527-Humans,
pubmed-meshheading:2170527-Interferon-gamma,
pubmed-meshheading:2170527-Molecular Weight,
pubmed-meshheading:2170527-Monocytes,
pubmed-meshheading:2170527-Precipitin Tests,
pubmed-meshheading:2170527-Receptors, Cell Surface,
pubmed-meshheading:2170527-Receptors, Immunologic,
pubmed-meshheading:2170527-Receptors, Interferon,
pubmed-meshheading:2170527-Receptors, Tumor Necrosis Factor,
pubmed-meshheading:2170527-Recombinant Proteins,
pubmed-meshheading:2170527-Tumor Necrosis Factor-alpha
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pubmed:year |
1990
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pubmed:articleTitle |
Regulation of IFN-gamma-receptor expression in human monocytes by granulocyte-macrophage colony-stimulating factor.
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pubmed:affiliation |
Klinische Arbeitsgruppe der Max-Planck-Gesellschaft, University of Göttingen, F.R.G.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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