| pubmed-article:2169925 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0006675 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0005955 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0013725 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0022022 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0033414 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0030685 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0031586 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0680255 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C0391871 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C1283071 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C1963578 | lld:lifeskim |
| pubmed-article:2169925 | lifeskim:mentions | umls-concept:C1514485 | lld:lifeskim |
| pubmed-article:2169925 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:2169925 | pubmed:dateCreated | 1990-11-19 | lld:pubmed |
| pubmed-article:2169925 | pubmed:abstractText | Eicosanoid release during multilineage hematopoiesis was assessed using freshly isolated mouse bone marrow cells cultured in the presence of interleukin-3 (IL-3) (10% WEHI-3 culture-conditioned medium). Cells that could release prostaglandin E2 (PGE2) when stimulated with calcium ionophore A23187, but not with phorbol ester (PMA), appeared within 4 days. The cells harvested on day 10 released 42 ng of PGE2/10(6) cells/mL after A23187 stimulation. Leukotriene B4 (LTB4) (4 ng/mL) was also detected after A23187 stimulation, but there was no detectable LTC4 (less than 0.5 ng/mL). Nonadherent bone marrow cells were isolated from 28-day cultures and cloned. All clones were strongly IL-3-dependent. Although other growth factors such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF), and CSF-1 failed to promote survival or support proliferation of the cells, three clones (11-1-A6, 3-2-D5, and 11-1-A1) showed significant increases in 3H-thymidine incorporation, respectively, after PMA treatment for 24 hours. Surviving cells displayed dominantly myeloid type morphology and phenotypic characteristics. The data suggest that IL-3 is important in the formation of PGE2-producing cells. In contrast to many macrophages (MO), neither the IL-3-dependent cell lines nor the IL-3-cultured bone marrow cells released significant amounts of PGE2 when stimulated with PMA or IL-3, although PMA and IL-3 both induced translocation of protein kinase C (PKC) to the membrane fraction. The lack of production of PGE2 and other eicosanoids by the PMA- and IL-3-stimulated cell lines was confirmed by measuring the release of 3H-arachidonic acid. The data suggest that in IL-3-dependent bone marrow cell lines the activation of eicosanoid metabolism requires elevated cellular Ca2+; PKC activation alone does not appear to be a sufficient stimulus. | lld:pubmed |
| pubmed-article:2169925 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:language | eng | lld:pubmed |
| pubmed-article:2169925 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:2169925 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2169925 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2169925 | pubmed:issn | 0006-4971 | lld:pubmed |
| pubmed-article:2169925 | pubmed:author | pubmed-author:SatoHH | lld:pubmed |
| pubmed-article:2169925 | pubmed:author | pubmed-author:ShibataYY | lld:pubmed |
| pubmed-article:2169925 | pubmed:author | pubmed-author:OghisoYY | lld:pubmed |
| pubmed-article:2169925 | pubmed:author | pubmed-author:McCaffreyP... | lld:pubmed |
| pubmed-article:2169925 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2169925 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:2169925 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:2169925 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2169925 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2169925 | pubmed:pagination | 1586-92 | lld:pubmed |
| pubmed-article:2169925 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:2169925 | pubmed:meshHeading | pubmed-meshheading:2169925-... | lld:pubmed |
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| pubmed-article:2169925 | pubmed:meshHeading | pubmed-meshheading:2169925-... | lld:pubmed |
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| pubmed-article:2169925 | pubmed:meshHeading | pubmed-meshheading:2169925-... | lld:pubmed |
| pubmed-article:2169925 | pubmed:meshHeading | pubmed-meshheading:2169925-... | lld:pubmed |
| pubmed-article:2169925 | pubmed:meshHeading | pubmed-meshheading:2169925-... | lld:pubmed |
| pubmed-article:2169925 | pubmed:meshHeading | pubmed-meshheading:2169925-... | lld:pubmed |
| pubmed-article:2169925 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2169925 | pubmed:articleTitle | Calcium ionophore but not phorbol ester promotes eicosanoids release by proliferating interleukin-3-dependent bone marrow cells. | lld:pubmed |
| pubmed-article:2169925 | pubmed:affiliation | Fujisaki Cell Center, Hayashibara Biochemical Laboratories Inc., Okayama, Japan. | lld:pubmed |
| pubmed-article:2169925 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2169925 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:2169925 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
