Linked Life Data

2169925

Source:http://linkedlifedata.com/resource/pubmed/id/2169925

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1990-11-19
pubmed:abstractText
Eicosanoid release during multilineage hematopoiesis was assessed using freshly isolated mouse bone marrow cells cultured in the presence of interleukin-3 (IL-3) (10% WEHI-3 culture-conditioned medium). Cells that could release prostaglandin E2 (PGE2) when stimulated with calcium ionophore A23187, but not with phorbol ester (PMA), appeared within 4 days. The cells harvested on day 10 released 42 ng of PGE2/10(6) cells/mL after A23187 stimulation. Leukotriene B4 (LTB4) (4 ng/mL) was also detected after A23187 stimulation, but there was no detectable LTC4 (less than 0.5 ng/mL). Nonadherent bone marrow cells were isolated from 28-day cultures and cloned. All clones were strongly IL-3-dependent. Although other growth factors such as granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage CSF (GM-CSF), and CSF-1 failed to promote survival or support proliferation of the cells, three clones (11-1-A6, 3-2-D5, and 11-1-A1) showed significant increases in 3H-thymidine incorporation, respectively, after PMA treatment for 24 hours. Surviving cells displayed dominantly myeloid type morphology and phenotypic characteristics. The data suggest that IL-3 is important in the formation of PGE2-producing cells. In contrast to many macrophages (MO), neither the IL-3-dependent cell lines nor the IL-3-cultured bone marrow cells released significant amounts of PGE2 when stimulated with PMA or IL-3, although PMA and IL-3 both induced translocation of protein kinase C (PKC) to the membrane fraction. The lack of production of PGE2 and other eicosanoids by the PMA- and IL-3-stimulated cell lines was confirmed by measuring the release of 3H-arachidonic acid. The data suggest that in IL-3-dependent bone marrow cell lines the activation of eicosanoid metabolism requires elevated cellular Ca2+; PKC activation alone does not appear to be a sufficient stimulus.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0006-4971
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1586-92
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:2169925-Animals, pubmed-meshheading:2169925-Arachidonic Acid, pubmed-meshheading:2169925-Arachidonic Acids, pubmed-meshheading:2169925-Bone Marrow Cells, pubmed-meshheading:2169925-Calcimycin, pubmed-meshheading:2169925-Calcium, pubmed-meshheading:2169925-Cell Division, pubmed-meshheading:2169925-Clone Cells, pubmed-meshheading:2169925-DNA, pubmed-meshheading:2169925-Dinoprostone, pubmed-meshheading:2169925-Female, pubmed-meshheading:2169925-Hematopoietic Stem Cells, pubmed-meshheading:2169925-Interleukin-3, pubmed-meshheading:2169925-Leukotriene B4, pubmed-meshheading:2169925-Macrophage Colony-Stimulating Factor, pubmed-meshheading:2169925-Macrophages, pubmed-meshheading:2169925-Mice, pubmed-meshheading:2169925-Mice, Inbred CBA, pubmed-meshheading:2169925-Protein Kinase C, pubmed-meshheading:2169925-Tetradecanoylphorbol Acetate
pubmed:year
1990
pubmed:articleTitle
Calcium ionophore but not phorbol ester promotes eicosanoids release by proliferating interleukin-3-dependent bone marrow cells.
pubmed:affiliation
Fujisaki Cell Center, Hayashibara Biochemical Laboratories Inc., Okayama, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't