Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1990-11-19
pubmed:abstractText
The potential of mouse monoclonal antibodies for recognising different antigens associated with human small cell lung cancer (SCLC) to form active immunotoxins was assessed by an indirect in vitro screening assay. The screening agent used was a conjugate made by linking ricin A chain to a sheep anti-mouse IgG Fab' fragment via a disulphide bond. The monoclonal antibodies SWA11 and SWA20 both mediated the toxic effects of ricin A chain against the HC12 classic SCLC cell line in dose-dependent fashion. The SWA11 antibody was the more effective; in combination with the screening agent at a concentration of 1 x 10(-7) M, it inhibited the incorporation of [3H] leucine into HC12 cells by 94% compared with only 44% inhibition in the case of SWA20. An immunotoxin made by the direct chemical conjugation of ricin A chain to SWA11 exhibited selective toxic effects upon HC12 cells in tissue culture inhibiting the incorporation of [3H] leucine by 50% at a concentration (IC50) of 6.2 x 10(-10) M and by 98% at 1 x 10(-7) M. SWA11-ricin A chain had an IC50 of 4.4 x 10(-10) M against the NCI-H69 classic SCLC cell line but showed no cytotoxic activity against the human lung adenocarcinoma cell line NCI-H23 at a concentration of 1 x 10(-8) M.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2153448, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2461993, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2539173, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2544218, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2822071, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2822229, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2839290, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-2985257, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-3257712, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-3900635, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-6318313, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-6544125, http://linkedlifedata.com/resource/pubmed/commentcorrection/2169852-7274624
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
410-4
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Selective cytotoxic effects of a ricin A chain immunotoxin made with the monoclonal antibody SWA11 recognising a human small cell lung cancer antigen.
pubmed:affiliation
Drug Targeting Laboratory, Institute of Cancer Research, Sutton, Surrey, UK.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't