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rdf:type |
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lifeskim:mentions |
umls-concept:C0004391,
umls-concept:C0037083,
umls-concept:C0086597,
umls-concept:C0243125,
umls-concept:C0699900,
umls-concept:C0851285,
umls-concept:C1414201,
umls-concept:C1414902,
umls-concept:C1704259,
umls-concept:C1705987,
umls-concept:C1710082
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pubmed:issue |
5
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pubmed:dateCreated |
2011-6-21
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pubmed:abstractText |
Wnt signaling is critical for many biological processes and is tightly regulated. In this study, we found that GABARAPL1 (GABA(A) receptor-associated protein like 1, GABARAPL1) interacts with Dvl2 by both yeast two-hybrid screening and immunoprecipitation experiments. Furthermore, we observed that p62 is required for the interaction of Dvl2 and GABARAPL1. Luciferase assays indicated that GABARAPL1 represses Wnt/?-catenin signaling stimulated by Wnt1, Dvl2 and ?-catenin. We further demonstrated that GABARAPL1 mediates degradation of Dvl2 and the effect is blocked by addition of 3-MA, a specific inhibitor of autophagy. Finally, we provided evidence that over-expression of GABARAPL1 inhibits proliferation and tumor growth of MCF7 cells in vitro and in nude mice. Taken together, our results suggested that GABARAPL1 as a tumor repressor inhibits Wnt signaling via mediating Dvl2 degradation through the autophagy pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Gabarapl1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Microtubule-Associated Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/WNT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt1 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/dishevelled proteins
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pubmed:status |
MEDLINE
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pubmed:issn |
1421-9778
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pubmed:author |
pubmed-author:ChangZhijieZ,
pubmed-author:JiaBaoqingB,
pubmed-author:LiangHanH,
pubmed-author:RenFangliF,
pubmed-author:SevyJJ,
pubmed-author:WangDianjunD,
pubmed-author:WangFangF,
pubmed-author:WuYinyuanY,
pubmed-author:XiaYongjingY,
pubmed-author:YangXiX,
pubmed-author:YilmazZekiZ,
pubmed-author:ZhaiYonggongY,
pubmed-author:ZhangYanquanY
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pubmed:copyrightInfo |
Copyright © 2011 S. Karger AG, Basel.
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pubmed:issnType |
Electronic
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
503-12
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pubmed:meshHeading |
pubmed-meshheading:21691068-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:21691068-Animals,
pubmed-meshheading:21691068-Autophagy,
pubmed-meshheading:21691068-Breast Neoplasms,
pubmed-meshheading:21691068-Cell Line, Tumor,
pubmed-meshheading:21691068-Cell Proliferation,
pubmed-meshheading:21691068-Cloning, Molecular,
pubmed-meshheading:21691068-Female,
pubmed-meshheading:21691068-Gene Expression,
pubmed-meshheading:21691068-HEK293 Cells,
pubmed-meshheading:21691068-Humans,
pubmed-meshheading:21691068-Immunoprecipitation,
pubmed-meshheading:21691068-Luciferases,
pubmed-meshheading:21691068-Mice,
pubmed-meshheading:21691068-Mice, Nude,
pubmed-meshheading:21691068-Microtubule-Associated Proteins,
pubmed-meshheading:21691068-Neoplasm Transplantation,
pubmed-meshheading:21691068-Phosphoproteins,
pubmed-meshheading:21691068-Plasmids,
pubmed-meshheading:21691068-Protein Binding,
pubmed-meshheading:21691068-Signal Transduction,
pubmed-meshheading:21691068-Transfection,
pubmed-meshheading:21691068-Two-Hybrid System Techniques,
pubmed-meshheading:21691068-Up-Regulation,
pubmed-meshheading:21691068-Uterine Cervical Neoplasms,
pubmed-meshheading:21691068-Wnt1 Protein,
pubmed-meshheading:21691068-beta Catenin
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pubmed:year |
2011
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pubmed:articleTitle |
GABARAPL1 negatively regulates Wnt/?-catenin signaling by mediating Dvl2 degradation through the autophagy pathway.
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pubmed:affiliation |
State Key Laboratory of Biomembrane and Membrane Biotechnology, School of Medicine, National Engineering Laboratory for Anti-tumor Therapeutics, Tsinghua University, Beijing, R.P. China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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