| pubmed-article:2168979 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:2168979 | lifeskim:mentions | umls-concept:C0369343 | lld:lifeskim |
| pubmed-article:2168979 | lifeskim:mentions | umls-concept:C1167117 | lld:lifeskim |
| pubmed-article:2168979 | lifeskim:mentions | umls-concept:C0598312 | lld:lifeskim |
| pubmed-article:2168979 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:2168979 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:2168979 | pubmed:dateCreated | 1990-10-17 | lld:pubmed |
| pubmed-article:2168979 | pubmed:abstractText | Proteins from herpes simplex virus (HSV)-infected cells were used to reconstitute DNA synthesis in vitro on a preformed replication fork. The preformed replication fork consisted of a nicked, double-stranded, circular DNA molecule with a 5' single-strand tail that was noncomplementary to the template. The products of DNA synthesis on this substrate were rolling-circle molecules, as demonstrated by electron microscopy and alkaline agarose gel electrophoresis. The tails contained double-stranded regions, indicating that both leading- and lagging-strand DNA syntheses occurred. Rolling-circle DNA replication was dependent upon HSV DNA polymerase and ATP and was stimulated by a crude fraction containing ICP8 (HSV DNA-binding protein). Similar protein fractions from mock-infected cells were unable to support rolling-circle DNA replication. This in vitro DNA replication system should prove useful in the identification and characterization of the enzymatic activities required at the HSV replication fork. | lld:pubmed |
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| pubmed-article:2168979 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:2168979 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:2168979 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:2168979 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:2168979 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:2168979 | pubmed:author | pubmed-author:RabkinS DSD | lld:pubmed |
| pubmed-article:2168979 | pubmed:author | pubmed-author:HanlonBB | lld:pubmed |
| pubmed-article:2168979 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:2168979 | pubmed:volume | 64 | lld:pubmed |
| pubmed-article:2168979 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:2168979 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:2168979 | pubmed:pagination | 4957-67 | lld:pubmed |
| pubmed-article:2168979 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:2168979 | pubmed:year | 1990 | lld:pubmed |
| pubmed-article:2168979 | pubmed:articleTitle | Herpes simplex virus DNA synthesis at a preformed replication fork in vitro. | lld:pubmed |
| pubmed-article:2168979 | pubmed:affiliation | Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021. | lld:pubmed |
| pubmed-article:2168979 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:2168979 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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