|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
10
|
|
pubmed:dateCreated |
1990-10-17
|
|
pubmed:abstractText |
Proteins from herpes simplex virus (HSV)-infected cells were used to reconstitute DNA synthesis in vitro on a preformed replication fork. The preformed replication fork consisted of a nicked, double-stranded, circular DNA molecule with a 5' single-strand tail that was noncomplementary to the template. The products of DNA synthesis on this substrate were rolling-circle molecules, as demonstrated by electron microscopy and alkaline agarose gel electrophoresis. The tails contained double-stranded regions, indicating that both leading- and lagging-strand DNA syntheses occurred. Rolling-circle DNA replication was dependent upon HSV DNA polymerase and ATP and was stimulated by a crude fraction containing ICP8 (HSV DNA-binding protein). Similar protein fractions from mock-infected cells were unable to support rolling-circle DNA replication. This in vitro DNA replication system should prove useful in the identification and characterization of the enzymatic activities required at the HSV replication fork.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-14033128,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-147874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-171843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-195088,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-199849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-21304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-219240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2538835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2545703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2547799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2547992,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2552170,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2555539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2578573,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2647732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2674143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2823462,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2824502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2826806,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2828677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2838481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2838482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2839594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-2840659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-3018724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-3024166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-3031067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-3033271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-3065520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-3084491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-3316214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-381293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-4304099,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-4335659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-4354205,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6103541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6115860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6257822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6270358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6311955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6329712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6360204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6597749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6803240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168979-6885816
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
64
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4957-67
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:2168979-Acyclovir,
pubmed-meshheading:2168979-Animals,
pubmed-meshheading:2168979-Antiviral Agents,
pubmed-meshheading:2168979-Cell Nucleus,
pubmed-meshheading:2168979-Cell Transformation, Viral,
pubmed-meshheading:2168979-DNA, Viral,
pubmed-meshheading:2168979-DNA Replication,
pubmed-meshheading:2168979-DNA-Directed DNA Polymerase,
pubmed-meshheading:2168979-Kinetics,
pubmed-meshheading:2168979-Microscopy, Electron,
pubmed-meshheading:2168979-Oligonucleotide Probes,
pubmed-meshheading:2168979-Simplexvirus,
pubmed-meshheading:2168979-Vero Cells
|
|
pubmed:year |
1990
|
|
pubmed:articleTitle |
Herpes simplex virus DNA synthesis at a preformed replication fork in vitro.
|
|
pubmed:affiliation |
Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
