Linked Life Data

2168847

Source:http://linkedlifedata.com/resource/pubmed/id/2168847

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1990-10-18
pubmed:abstractText
The Escherichia coli rRNA operons each have one of two types of spacer separating the 16S and 23S coding regions. The spacers of four operons encode tRNA(Glu2) and the other three encode both tRNA(Ile) and tRNA(Ala1B). We have prepared a series of mutants in which the spacer region of a particular rrn operon has been replaced by the opposite type. Included among these were a mutant retaining only a single copy of the tRNA(Glu2) spacer (at rrnG) and another retaining only a single copy of the tRNA(Ile)-tRNA(Ala1B) spacer (at rrnA). While both mutants grew more slowly than controls, the mutant deficient in tRNA(Glu2) spacers was more severely affected. At a frequency of 6 X 10(-5), these mutants phenotypically reverted to faster growing types by increasing the copy number of the deficient spacer. In most of these phenotypic revertants, the deficient spacer type appeared in a rrn operon which previously contained the surplus type, bringing the ratio of spacer types closer to normal. In a few cases, these spacer changes were accompanied by an inversion of the chromosomal material between the donor and recipient rrn operons. Two examples of inversion of one-half of the E. coli chromosome between rrnG and rrnH were observed. The correlation of spacer change with inversion indicated that, in these particular cases, the change was due to an intrachromatid gene conversion event accompanied by a reciprocal crossover rather than reciprocal exchange between sister chromatids.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-16562114, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-2843741, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-3007275, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-3161076, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-3277949, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-360975, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-377232, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-389624, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-4361142, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-4903099, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-4923751, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-4935283, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-6094483, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-6173373, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-6285294, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-6299577, http://linkedlifedata.com/resource/pubmed/commentcorrection/2168847-7028991
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0016-6731
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
683-90
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1990
pubmed:articleTitle
Exchange of spacer regions between rRNA operons in Escherichia coli.
pubmed:affiliation
Department of Biological Chemistry, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.