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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
28
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pubmed:dateCreated |
1990-10-12
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pubmed:abstractText |
The dynamics of the enthalpy and volume changes found in the photodissociation of CO from sperm whale carboxymyoglobin and two site-directed mutants in which arginine-45 is replaced by glycine and asparagine are examined by photoacoustic calorimetry. An intermediate is observed whose lifetime at 20 degrees C is 700 ns. The enthalpy of the intermediate increases by approximately 7 kcal/mol upon replacing arginine-45 with either asparagine or glycine. These observations support recent proposals that an arginine-45 salt bridge is broken upon ligand dissociation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6741-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:2168740-Acoustics,
pubmed-meshheading:2168740-Animals,
pubmed-meshheading:2168740-Arginine,
pubmed-meshheading:2168740-Base Sequence,
pubmed-meshheading:2168740-Calorimetry,
pubmed-meshheading:2168740-Carbon Monoxide,
pubmed-meshheading:2168740-Chemistry, Physical,
pubmed-meshheading:2168740-Diffusion,
pubmed-meshheading:2168740-Molecular Sequence Data,
pubmed-meshheading:2168740-Mutation,
pubmed-meshheading:2168740-Myoglobin,
pubmed-meshheading:2168740-Oligodeoxyribonucleotides,
pubmed-meshheading:2168740-Photochemistry,
pubmed-meshheading:2168740-Physicochemical Phenomena,
pubmed-meshheading:2168740-Protein Engineering,
pubmed-meshheading:2168740-Protons,
pubmed-meshheading:2168740-Recombinant Fusion Proteins,
pubmed-meshheading:2168740-Whales
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pubmed:year |
1990
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pubmed:articleTitle |
Role of the arginine-45 salt bridge in ligand dissociation from sperm whale carboxymyoglobin as probed by photoacoustic calorimetry.
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pubmed:affiliation |
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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