Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1990-10-11
|
| pubmed:abstractText |
Studies of chromatin structure were performed in mouse fibroblast cell lines containing Bovine Papilloma Virus (BPV) based artificial minichromosomes containing Mouse Mammary Tumor Virus (MMTV) Long Terminal Repeat (LTR), a retroviral promoter regulated by glucocorticoids, driving the transcription of v-Ha-ras. These minichromosomes fractionate with the "active chromatin", indicating an association of the minichromosomes with components of the "nuclear matrix". Two regions of the minichromosomes upstream and downstream of v-Ha-ras are involved in this interaction. MMTV LTR promoter is associated with nucleosomes precisely positioned on the DNA sequences. Hormonal activation is accompanied by a structural change of the nucleosome associated with the hormone response elements (HREs). This structural change can be visualized by the appearance of a hormono-dependent DNaseI hypersensitive site. Anti-hormones, even when able to promote a strong binding of the receptor to the nucleus, are unable to induce the chromatin structural change. The strong association of the hormone-receptor complex with the nucleus is necessary to induce the DNaseI hypersensitive site and to maintain the transcription, but is not necessary for DNaseI hypersensitivity maintenance. This suggests a double role for the hormone-receptor complex: 1) induction of a chromatin rearrangement and 2) transcriptional transactivation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Recombinant,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleosomes,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Protein p21(ras),
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucocorticoid,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/dexamethasone receptor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0034-9402
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
46
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
31-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:2168572-Animals,
pubmed-meshheading:2168572-Binding Sites,
pubmed-meshheading:2168572-Cells, Cultured,
pubmed-meshheading:2168572-Chromatin,
pubmed-meshheading:2168572-DNA, Recombinant,
pubmed-meshheading:2168572-Dexamethasone,
pubmed-meshheading:2168572-Gene Expression Regulation, Viral,
pubmed-meshheading:2168572-Genes, Synthetic,
pubmed-meshheading:2168572-Genes, Viral,
pubmed-meshheading:2168572-Genetic Vectors,
pubmed-meshheading:2168572-Mammary Tumor Virus, Mouse,
pubmed-meshheading:2168572-Mice,
pubmed-meshheading:2168572-Molecular Conformation,
pubmed-meshheading:2168572-Nucleosomes,
pubmed-meshheading:2168572-Oncogene Protein p21(ras),
pubmed-meshheading:2168572-Promoter Regions, Genetic,
pubmed-meshheading:2168572-Receptors, Glucocorticoid,
pubmed-meshheading:2168572-Recombinant Fusion Proteins,
pubmed-meshheading:2168572-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:2168572-Transcription, Genetic
|
| pubmed:year |
1990
|
| pubmed:articleTitle |
Role of chromatin structure in transcriptional regulation of MMTV LTR hormone-dependent promoter.
|
| pubmed:affiliation |
Unité de Recherches sur les communications hormonales, INSERM U-33, Hôpital du Kremlin Bicêtre, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|